Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1453837..1454438 Replicon chromosome
Accession NZ_CP028948
Organism Serratia marcescens strain AR_0123

Toxin (Protein)


Gene name vapC Uniprot ID A0A2V4FS51
Locus tag AM370_RS07025 Protein ID WP_033649548.1
Coordinates 1454058..1454438 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A086G6A0
Locus tag AM370_RS07020 Protein ID WP_004933932.1
Coordinates 1453837..1454058 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM370_RS07005 1448985..1449962 + 978 WP_033636542.1 glyoxylate/hydroxypyruvate reductase GhrB -
AM370_RS24545 1449964..1450116 - 153 WP_019455508.1 hypothetical protein -
AM370_RS07010 1450313..1452376 + 2064 WP_048321223.1 alpha-amylase -
AM370_RS07015 1452512..1453762 + 1251 WP_033649547.1 valine--pyruvate transaminase -
AM370_RS07020 1453837..1454058 + 222 WP_004933932.1 ribbon-helix-helix domain-containing protein Antitoxin
AM370_RS07025 1454058..1454438 + 381 WP_033649548.1 type II toxin-antitoxin system VapC family toxin Toxin
AM370_RS07030 1454449..1456107 - 1659 WP_033649549.1 putative transporter -
AM370_RS07035 1456274..1456702 - 429 WP_004933922.1 heat shock chaperone IbpB -
AM370_RS07040 1456803..1457216 - 414 WP_004933919.1 heat shock chaperone IbpA -
AM370_RS07045 1457527..1457874 + 348 WP_033636538.1 YceK/YidQ family lipoprotein -
AM370_RS07050 1457905..1459185 - 1281 WP_033649550.1 DUF3748 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-102)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-40)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14171.50 Da        Isoelectric Point: 7.3170

>T104436 WP_033649548.1 NZ_CP028948:1454058-1454438 [Serratia marcescens]
MVAQRALFDTNILIDYLNGIPQAKDVLTEYHINPAISAITWMEVMVGAKKQGPALELKTRQFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIVWATAQVGFRTLISRNPKDFGTDNGVLMPYRL

Download         Length: 381 bp

>T104436 NZ_CP028948:1454058-1454438 [Serratia marcescens]
ATGGTGGCTCAACGGGCGTTATTCGACACCAATATCCTGATCGATTATCTCAACGGTATTCCTCAAGCCAAAGACGTTCT
GACCGAATACCACATCAACCCGGCTATTAGCGCCATCACCTGGATGGAAGTGATGGTGGGTGCCAAAAAACAGGGGCCGG
CGCTGGAGCTGAAAACGCGCCAGTTTTTAGGGCAATTCCTGCTGCTGCCTATTACCGATGAAGTTGCCGAACGCGCCGTA
GAACTGCGCCACTCACAGCATGTGAAGCTGCCGGACGCCATTGTCTGGGCCACCGCGCAGGTGGGGTTCCGGACGCTCAT
CTCCCGCAACCCCAAAGATTTTGGCACTGACAACGGCGTGTTAATGCCTTATCGGCTGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8593.53 Da        Isoelectric Point: 4.4791

>AT104436 WP_004933932.1 NZ_CP028948:1453837-1454058 [Serratia marcescens]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVDYENRQREEWE

Download         Length: 222 bp

>AT104436 NZ_CP028948:1453837-1454058 [Serratia marcescens]
ATGACTCGCATCGTGATCGATTTACCTGAAGAAGACCTGCGGCAGCTCGACAATCTGAAAAATATCCGCCACGTTCCCCG
GGCCGAAATCATCCGTCAGGCCGTCTCCCGTTATCTGACGGAGAACCACGTTGAAGATCCCAGCAATGCTTTCGGGCTCT
GGTCCGGGGCGATGGGCGATGGCGTAGACTACGAAAATCGTCAGCGCGAGGAGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V4FS51

Antitoxin

Source ID Structure
AlphaFold DB A0A086G6A0

References