Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3153315..3153868 Replicon chromosome
Accession NZ_CP028946
Organism Serratia marcescens strain AR_0124

Toxin (Protein)


Gene name ccdB Uniprot ID A0A2V4FZ31
Locus tag AM371_RS14965 Protein ID WP_019454426.1
Coordinates 3153554..3153868 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A2V4GJ62
Locus tag AM371_RS14960 Protein ID WP_029988820.1
Coordinates 3153315..3153551 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM371_RS14945 3149782..3151317 + 1536 WP_033646792.1 glutathione ABC transporter substrate-binding protein GsiB -
AM371_RS14950 3151370..3152290 + 921 WP_033646790.1 glutathione ABC transporter permease GsiC -
AM371_RS14955 3152300..3153208 + 909 WP_172448764.1 glutathione ABC transporter permease GsiD -
AM371_RS14960 3153315..3153551 + 237 WP_029988820.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
AM371_RS14965 3153554..3153868 + 315 WP_019454426.1 CcdB family protein Toxin
AM371_RS14970 3153908..3154750 - 843 WP_015377214.1 S-formylglutathione hydrolase -
AM371_RS14975 3154765..3155889 - 1125 WP_033646788.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
AM371_RS14980 3155920..3156840 - 921 WP_016928453.1 LysR family transcriptional regulator -
AM371_RS14985 3156946..3158103 + 1158 WP_033646786.1 YbfB/YjiJ family MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11745.68 Da        Isoelectric Point: 5.0488

>T104421 WP_019454426.1 NZ_CP028946:3153554-3153868 [Serratia marcescens]
MQFTVYANRGNSAVYPLLLDVTSDIIGQLNRRVVIPLLPVEKYPGSTRPERLIPLIRLIDDNEYAVMTYEMASIPVRALG
AEFCDVSQYRTRIKAAIDFLLDGI

Download         Length: 315 bp

>T104421 NZ_CP028946:3153554-3153868 [Serratia marcescens]
ATGCAATTTACGGTGTATGCGAATAGGGGGAACAGCGCGGTTTATCCCCTGCTGCTTGATGTGACGAGCGATATCATTGG
GCAATTAAATCGTCGAGTTGTGATCCCTTTGTTGCCCGTTGAAAAATACCCGGGGAGTACGCGACCGGAACGCTTGATCC
CGTTGATCAGATTGATTGATGACAACGAGTACGCCGTGATGACATATGAAATGGCCAGCATCCCGGTTCGGGCTTTGGGG
GCCGAGTTTTGTGATGTTTCCCAGTATCGGACCAGGATAAAAGCCGCCATCGACTTTCTTCTTGACGGTATTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9082.13 Da        Isoelectric Point: 5.7070

>AT104421 WP_029988820.1 NZ_CP028946:3153315-3153551 [Serratia marcescens]
MPVKQRTTQSVTMTLERSLLSRAREAGINLSATLTAALDAELRQHERIKWQEENREALEALNRFNDEHGCFSDEYRTF

Download         Length: 237 bp

>AT104421 NZ_CP028946:3153315-3153551 [Serratia marcescens]
ATGCCAGTAAAACAACGAACGACGCAAAGTGTGACCATGACGCTGGAACGCAGTTTGTTGAGCCGTGCGCGTGAGGCGGG
GATTAATCTCAGCGCGACGCTAACTGCTGCTTTAGATGCTGAGTTACGTCAGCATGAGAGGATAAAATGGCAGGAAGAAA
ACCGAGAGGCGCTTGAAGCGCTTAACCGATTTAATGATGAGCATGGTTGCTTTAGCGACGAATACAGGACGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2V4FZ31

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4GJ62

References