Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33923..34566 Replicon plasmid unnamed3
Accession NZ_CP028931
Organism Klebsiella pneumoniae strain AR_0153

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag AM400_RS02495 Protein ID WP_001044770.1
Coordinates 33923..34339 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag AM400_RS02500 Protein ID WP_001261282.1
Coordinates 34336..34566 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM400_RS02465 29091..29879 + 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
AM400_RS02470 29939..30763 + 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
AM400_RS02475 30963..31280 + 318 Protein_34 recombinase family protein -
AM400_RS02480 31463..32323 + 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
AM400_RS02485 32408..33105 - 698 Protein_36 IS1 family transposase -
AM400_RS02490 33139..33849 - 711 WP_004152334.1 ATP-binding protein -
AM400_RS02495 33923..34339 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
AM400_RS02500 34336..34566 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AM400_RS29520 34523..34984 + 462 WP_072202616.1 hypothetical protein -
AM400_RS02510 35128..35478 + 351 WP_000493378.1 hypothetical protein -
AM400_RS02515 35529..36272 + 744 WP_000129823.1 hypothetical protein -
AM400_RS02520 36269..37045 + 777 WP_000015958.1 site-specific integrase -
AM400_RS02525 37103..37360 - 258 WP_000764642.1 hypothetical protein -
AM400_RS29525 37489..37593 - 105 WP_032409716.1 hypothetical protein -
AM400_RS02530 38128..38994 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ere(A) / ARR-3 / blaSHV-187 / aac(3)-IId / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / cmlA1 - 1..70829 70829
- inside Integron - - 1..70829 70828


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T104384 WP_001044770.1 NZ_CP028931:c34339-33923 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T104384 NZ_CP028931:c34339-33923 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT104384 WP_001261282.1 NZ_CP028931:c34566-34336 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT104384 NZ_CP028931:c34566-34336 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References