Detailed information of TA system TA104382

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 101723..102474
• Replicon: plasmid unnamed1
• Accession: NZ_CP028929
• Organism: Klebsiella pneumoniae strain AR_0153

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: AM400_RS00685
• Protein ID: WP_014386536.1
• Coordinates: 101992..102474 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: AM400_RS00680
• Protein ID: WP_004902250.1
• Coordinates: 101723..102001 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM400_RS00640	97366..97791	-	426	WP_000522996.1	organomercurial transporter MerC	-
AM400_RS00645	97819..98094	-	276	WP_043907009.1	mercury resistance system periplasmic binding protein MerP	-
AM400_RS00650	98110..98475	-	366	WP_004200999.1	mercuric ion transporter MerT	-
AM400_RS00655	98547..99002	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
AM400_RS00660	99655..100113	+	459	WP_014386535.1	hypothetical protein	-
AM400_RS00670	100943..101284	+	342	WP_004902257.1	hypothetical protein	-
AM400_RS00675	101392..101604	+	213	WP_004902255.1	hypothetical protein	-
AM400_RS00680	101723..102001	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
AM400_RS00685	101992..102474	+	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
AM400_RS00700	103509..104048	+	540	WP_004902239.1	hypothetical protein	-
AM400_RS00705	104153..104545	+	393	WP_032442757.1	hypothetical protein	-
AM400_RS00710	104646..105401	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
AM400_RS00715	105428..106075	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12	-	1..283371	283371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T104382 WP_014386536.1 NZ_CP028929:101992-102474 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T104382 NZ_CP028929:101992-102474 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT104382 WP_004902250.1 NZ_CP028929:101723-102001 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT104382 NZ_CP028929:101723-102001 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3