Detailed information of TA system TA104379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 440130..440770
• Replicon: chromosome
• Accession: NZ_CP028926
• Organism: Pasteurella multocida strain 20N

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V4NDJ2
• Locus tag: DB278_RS02015
• Protein ID: WP_016533221.1
• Coordinates: 440130..440306 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DB278_RS02020
• Protein ID: WP_108511501.1
• Coordinates: 440354..440770 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB278_RS01995	435531..437069	-	1539	WP_108511499.1	phage portal protein	-
DB278_RS02000	437066..437287	-	222	WP_014391481.1	hypothetical protein	-
DB278_RS02005	437284..439392	-	2109	WP_108511500.1	phage terminase large subunit family protein	-
DB278_RS02010	439395..439871	-	477	WP_061406081.1	DUF1441 family protein	-
DB278_RS02015	440130..440306	+	177	WP_016533221.1	type II toxin-antitoxin system HicA family toxin	Toxin
DB278_RS02020	440354..440770	+	417	WP_108511501.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DB278_RS02030	440994..441317	-	324	WP_108511503.1	DUF2570 family protein	-
DB278_RS02035	441290..441820	-	531	WP_108511504.1	lysozyme	-
DB278_RS02040	441817..442113	-	297	WP_081273045.1	hypothetical protein	-
DB278_RS02050	442366..442812	-	447	WP_108512040.1	antitermination protein	-
DB278_RS02055	442866..443447	-	582	WP_108511505.1	recombination protein NinG	-
DB278_RS02060	443521..443946	-	426	WP_108511506.1	recombination protein NinB	-
DB278_RS02065	443950..445386	-	1437	WP_108511507.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	406066..461125	55059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-78)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6530.60 Da
• Isoelectric Point: 10.7333

>T104379 WP_016533221.1 NZ_CP028926:440130-440306 [Pasteurella multocida]
MKQSEFLRWLKAQGVETKEGSNHIKLYLNGNQSALPRHPSKEIAKGTEIAVKKQLGLK

Nucleotide

• Length: 177 bp

>T104379 NZ_CP028926:440130-440306 [Pasteurella multocida]
ATGAAACAAAGTGAGTTTTTAAGATGGCTGAAAGCTCAAGGGGTAGAAACTAAAGAAGGCTCAAACCACATTAAGCTCTA
CCTAAACGGCAACCAATCAGCTCTCCCAAGACATCCGAGTAAAGAGATAGCAAAGGGAACTGAAATAGCAGTTAAAAAGC
AATTAGGTCTAAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15553.84 Da
• Isoelectric Point: 4.5390

>AT104379 WP_108511501.1 NZ_CP028926:440354-440770 [Pasteurella multocida]
MLYPAKFDKEDNGLYAVSFRDIPEALTCGDNFDDAVAMAKDALITSMDFYFEDFRKVPLPSEAKQDEVLIELPDSVFAKV
LLLNEMVEQNISNAELARRINVRPQEMQRITNISHSTKIDTISRALSALGKKLQLSVV

Nucleotide

• Length: 417 bp

>AT104379 NZ_CP028926:440354-440770 [Pasteurella multocida]
ATGTTATATCCAGCAAAATTTGATAAAGAAGATAACGGGCTTTATGCTGTATCATTTCGTGATATACCTGAAGCTTTAAC
TTGTGGTGATAACTTCGACGATGCCGTCGCAATGGCAAAAGATGCCTTGATTACATCAATGGATTTTTATTTTGAAGATT
TCCGCAAAGTTCCATTACCAAGTGAGGCAAAACAAGATGAAGTTTTAATTGAGTTACCAGATAGTGTTTTTGCTAAAGTG
CTTTTATTAAATGAGATGGTTGAACAAAATATTTCTAATGCTGAATTAGCAAGAAGAATTAATGTTCGACCACAAGAAAT
GCAACGTATTACTAATATCTCGCATAGCACAAAAATTGACACAATAAGCCGTGCTTTATCAGCACTCGGTAAAAAACTAC
AACTTTCAGTCGTCTAA

Structures

Toxin

• AlphaFold DB: V4NDJ2