Detailed information of TA system TA104369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4386416..4387458
• Replicon: chromosome
• Accession: NZ_CP028917
• Organism: Pseudomonas aeruginosa strain JB2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: C5B40_RS20805
• Protein ID: WP_003109777.1
• Coordinates: 4386416..4386991 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: C5B40_RS20810
• Protein ID: WP_003050245.1
• Coordinates: 4386988..4387458 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B40_RS20780	4382854..4383579	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
C5B40_RS20785	4383618..4384520	-	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
C5B40_RS20790	4384520..4385020	-	501	WP_003090159.1	hypothetical protein	-
C5B40_RS20795	4385017..4385487	-	471	WP_003105626.1	hypothetical protein	-
C5B40_RS20800	4385484..4386398	-	915	WP_003105629.1	AAA family ATPase	-
C5B40_RS20805	4386416..4386991	-	576	WP_003109777.1	PIN domain-containing protein	Toxin
C5B40_RS20810	4386988..4387458	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
C5B40_RS20815	4387662..4388045	+	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
C5B40_RS20820	4388042..4388275	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
C5B40_RS20825	4388292..4388651	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
C5B40_RS20830	4388663..4389061	+	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
C5B40_RS20835	4389058..4389750	+	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
C5B40_RS20840	4389747..4390658	+	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
C5B40_RS20845	4390648..4392066	+	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4336229..4433554	97325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T104369 WP_003109777.1 NZ_CP028917:c4386991-4386416 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T104369 NZ_CP028917:c4386991-4386416 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT104369 WP_003050245.1 NZ_CP028917:c4387458-4386988 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT104369 NZ_CP028917:c4387458-4386988 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68