Detailed information of TA system TA104365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 145436..145941
• Replicon: chromosome
• Accession: NZ_CP028917
• Organism: Pseudomonas aeruginosa strain JB2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: C5B40_RS00670
• Protein ID: WP_003083773.1
• Coordinates: 145436..145717 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: C5B40_RS00675
• Protein ID: WP_003083775.1
• Coordinates: 145714..145941 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5B40_RS00645	140687..142036	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
C5B40_RS00650	142085..142771	+	687	WP_003083762.1	FadR family transcriptional regulator	-
C5B40_RS00655	142872..143606	+	735	WP_003110658.1	GntR family transcriptional regulator	-
C5B40_RS00660	143810..144196	+	387	WP_003083767.1	aegerolysin family protein	-
C5B40_RS00665	144228..145136	-	909	WP_024947025.1	LysR family transcriptional regulator	-
C5B40_RS00670	145436..145717	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
C5B40_RS00675	145714..145941	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
C5B40_RS00680	146117..146737	-	621	WP_003101226.1	hypothetical protein	-
C5B40_RS00685	146838..147338	+	501	WP_003101228.1	LEA type 2 family protein	-
C5B40_RS00690	147411..147752	+	342	WP_024947024.1	alkylphosphonate utilization protein	-
C5B40_RS00695	147834..149261	-	1428	WP_023092260.1	GABA permease	-
C5B40_RS00700	149430..150923	-	1494	WP_024947023.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T104365 WP_003083773.1 NZ_CP028917:c145717-145436 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T104365 NZ_CP028917:c145717-145436 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT104365 WP_003083775.1 NZ_CP028917:c145941-145714 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT104365 NZ_CP028917:c145941-145714 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGGCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9