Detailed information of TA system TA104363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3047361..3047977
• Replicon: chromosome
• Accession: NZ_CP028908
• Organism: Bradyrhizobium sp. WD16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: DB459_RS14120
• Protein ID: WP_253713565.1
• Coordinates: 3047681..3047977 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: DB459_RS14115
• Protein ID: WP_253705837.1
• Coordinates: 3047361..3047678 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DB459_RS14090 (DB459_14050)	3043857..3044381	+	525	WP_253705825.1	PRC-barrel domain-containing protein	-
DB459_RS14095 (DB459_14055)	3044642..3044995	+	354	WP_253705827.1	cupredoxin domain-containing protein	-
DB459_RS14100 (DB459_14060)	3045006..3045851	+	846	WP_253705829.1	FTR1 family protein	-
DB459_RS14105 (DB459_14065)	3046128..3046562	-	435	WP_253705831.1	helix-turn-helix transcriptional regulator	-
DB459_RS14110	3046685..3047149	-	465	WP_253705835.1	hypothetical protein	-
DB459_RS14115 (DB459_14080)	3047361..3047678	-	318	WP_253705837.1	putative addiction module antidote protein	Antitoxin
DB459_RS14120 (DB459_14085)	3047681..3047977	-	297	WP_253713565.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DB459_RS14125 (DB459_14090)	3048033..3048314	-	282	Protein_2801	tyrosine-type recombinase/integrase	-
DB459_RS14130	3048505..3048792	-	288	WP_253705839.1	hypothetical protein	-
DB459_RS14135 (DB459_14095)	3048899..3049114	+	216	WP_253705841.1	hypothetical protein	-
DB459_RS14140	3049151..3049444	-	294	WP_253705843.1	hypothetical protein	-
DB459_RS27360	3049798..3049932	+	135	WP_256519169.1	hypothetical protein	-
DB459_RS14145 (DB459_14100)	3049939..3050223	+	285	WP_253705845.1	hypothetical protein	-
DB459_RS14150 (DB459_14105)	3050686..3051273	+	588	WP_253705847.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-94)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10900.38 Da
• Isoelectric Point: 9.9950

>T104363 WP_253713565.1 NZ_CP028908:c3047977-3047681 [Bradyrhizobium sp. WD16]
IIDVRETPDFTNWLAGLSDQRARLQIVRRISRVAAGNFGDAKSVGGAVSELRIDHGPGYRLYYTRRGKAVVILLCGGDKR
TQSKDIRKAQEIAETLED

Nucleotide

• Length: 297 bp

>T104363 NZ_CP028908:c3047977-3047681 [Bradyrhizobium sp. WD16]
ATCATCGACGTTCGCGAGACACCCGACTTCACAAACTGGCTTGCCGGCCTGAGCGATCAGCGCGCACGCTTGCAGATCGT
TCGCCGGATCAGCCGGGTTGCCGCTGGCAACTTCGGCGATGCGAAGTCGGTCGGAGGTGCGGTTAGCGAGCTTCGTATCG
ACCACGGCCCGGGATACCGGCTTTATTACACTCGGCGCGGCAAGGCCGTCGTCATTCTACTCTGCGGCGGCGACAAGCGC
ACCCAGAGTAAGGACATCCGGAAGGCCCAGGAAATCGCAGAGACATTGGAGGACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11269.14 Da
• Isoelectric Point: 10.3581

>AT104363 WP_253705837.1 NZ_CP028908:c3047678-3047361 [Bradyrhizobium sp. WD16]
MKVSKLKKFDAAEHLRTPEARAEYLNLVLADGDPAEVRDALNLVARAQGMSEVAKAAGVTREGLYKTLGENGNPEFATIL
RIIGAMGIRLVAEPARAKPKKKAVA

Nucleotide

• Length: 318 bp

>AT104363 NZ_CP028908:c3047678-3047361 [Bradyrhizobium sp. WD16]
ATGAAAGTCAGTAAACTCAAGAAGTTCGACGCCGCCGAGCACCTTCGCACACCGGAAGCACGTGCGGAGTATCTCAACCT
CGTTCTTGCCGATGGTGATCCCGCCGAAGTCCGCGACGCCCTCAATCTCGTCGCCCGAGCGCAGGGTATGAGCGAGGTTG
CCAAGGCTGCTGGCGTGACCAGAGAGGGGCTTTACAAGACGCTCGGCGAGAATGGCAACCCGGAATTCGCAACCATCCTG
CGGATCATCGGGGCCATGGGAATCCGGCTCGTAGCAGAACCGGCCCGAGCCAAGCCGAAAAAGAAGGCCGTGGCCTGA