Detailed information of TA system TA104333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/-
• Location: 467668..468452
• Replicon: chromosome
• Accession: NZ_CP028893
• Organism: Vibrio cholerae strain Sa5Y

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: Sa5Y_RS17030
• Protein ID: WP_001114075.1
• Coordinates: 467668..467937 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: Sa5Y_RS17035
• Protein ID: WP_094393031.1
• Coordinates: 467931..468452 (+)
• Length: 174 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Sa5Y_RS16995	463115..463540	+	426	WP_001898445.1	hypothetical protein	-
Sa5Y_RS17000	463775..464098	+	324	WP_001904612.1	DUF3709 domain-containing protein	-
Sa5Y_RS17005	464373..464870	+	498	WP_033933253.1	GNAT family N-acetyltransferase	-
Sa5Y_RS17015	465165..465347	+	183	Protein_529	DUF645 family protein	-
Sa5Y_RS17020	465589..466575	-	987	WP_094393029.1	sulfate ABC transporter substrate-binding protein	-
Sa5Y_RS17025	466837..467475	+	639	WP_094393030.1	HD domain-containing protein	-
Sa5Y_RS17030	467668..467937	+	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
Sa5Y_RS17035	467931..468452	+	522	WP_094393031.1	DUF2442 domain-containing protein	Antitoxin
Sa5Y_RS17040	468618..469358	+	741	WP_162298990.1	PhzF family phenazine biosynthesis protein	-
Sa5Y_RS17045	469653..469835	+	183	WP_094393107.1	DUF645 family protein	-
Sa5Y_RS17050	470033..470806	+	774	WP_094393066.1	aminoglycoside phosphotransferase family protein	-
Sa5Y_RS17055	470960..471313	+	354	WP_094393064.1	hypothetical protein	-
Sa5Y_RS19925	471297..471992	+	696	WP_128739199.1	hypothetical protein	-
Sa5Y_RS17065	472197..472460	+	264	WP_032473149.1	hypothetical protein	-
Sa5Y_RS17070	472686..472928	+	243	WP_033930503.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306104..472928	166824
	-	inside	Integron	-	-	307268..474111	166843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (1-173)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T104333 WP_001114075.1 NZ_CP028893:467668-467937 [Vibrio cholerae]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T104333 NZ_CP028893:467668-467937 [Vibrio cholerae]
ATGCCAGAGATTGATGCCCTATTAGGCCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTGCCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AGCGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin

Protein

• Length: 174 a.a.
• Molecular weight: 19455.10 Da
• Isoelectric Point: 6.7342

>AT104333 WP_094393031.1 NZ_CP028893:467931-468452 [Vibrio cholerae]
MLKVIDVDFVSDHTLELMFNDGYQGYADLSVYFKKTPFSEIKDFKRFSLTRDGSLNWDGNELSAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Nucleotide

• Length: 522 bp

>AT104333 NZ_CP028893:467931-468452 [Vibrio cholerae]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGATGTTCAATGATGGTTACCAAGGTTATGC
CGACTTGTCGGTATACTTTAAAAAAACACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CATTAAATTGGGATGGTAATGAGTTATCGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTAGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGCCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCAAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6