Detailed information of TA system TA104325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 400951..401498
• Replicon: chromosome
• Accession: NZ_CP028893
• Organism: Vibrio cholerae strain Sa5Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Sa5Y_RS16265
• Protein ID: WP_094393067.1
• Coordinates: 401196..401498 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: Sa5Y_RS16260
• Protein ID: WP_000861987.1
• Coordinates: 400951..401208 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Sa5Y_RS16215	396051..396368	-	318	WP_059261326.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Sa5Y_RS16220	396387..396629	-	243	WP_000288450.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
Sa5Y_RS16225	396890..397630	+	741	WP_162298990.1	PhzF family phenazine biosynthesis protein	-
Sa5Y_RS16230	397925..398107	+	183	WP_094393107.1	DUF645 family protein	-
Sa5Y_RS16235	398426..398704	+	279	WP_005526581.1	DUF3601 domain-containing protein	-
Sa5Y_RS16240	398739..399698	-	960	WP_094393086.1	IS30 family transposase	-
Sa5Y_RS16245	399853..399936	+	84	WP_080478874.1	acetyltransferase	-
Sa5Y_RS16250	399949..400308	+	360	WP_095488206.1	VOC family protein	-
Sa5Y_RS16260	400951..401208	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Sa5Y_RS16265	401196..401498	+	303	WP_094393067.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Sa5Y_RS16270	401656..402204	+	549	WP_094393068.1	hypothetical protein	-
Sa5Y_RS16275	402323..402694	+	372	WP_162298937.1	DUF4144 domain-containing protein	-
Sa5Y_RS16280	402969..403205	+	237	WP_161783514.1	DUF3709 domain-containing protein	-
Sa5Y_RS20300	403649..403820	+	172	Protein_417	DUF645 family protein	-
Sa5Y_RS16295	403970..404455	+	486	WP_175234067.1	GNAT family N-acetyltransferase	-
Sa5Y_RS16300	404628..405218	+	591	WP_094392960.1	TIR domain-containing protein	-
Sa5Y_RS16305	405401..406285	+	885	WP_103155538.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306104..472928	166824
	-	inside	Integron	-	-	307268..474111	166843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11793.73 Da
• Isoelectric Point: 5.1972

>T104325 WP_094393067.1 NZ_CP028893:401196-401498 [Vibrio cholerae]
MVEIIWTEPALSDLNDIAEYIALENVVAARQLVQTIFTKVERLADFPESGRIPPELEHLNYREVVVNPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T104325 NZ_CP028893:401196-401498 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCCGGCTCTATCCGATCTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
CGCTGCTAGACAATTGGTGCAAACCATTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAATCTGGACGTATCCCGC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT104325 WP_000861987.1 NZ_CP028893:400951-401208 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT104325 NZ_CP028893:400951-401208 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTATCTTATTGATGTAGACGACTATGAATTTATGCAAAACCGTTTAGCGA
TCCTGGAAGGTATTGCTCGTGGAGAGCGAGCTCTAGCGGATGGCAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4SLM9