Detailed information of TA system TA104322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 367889..368454
• Replicon: chromosome
• Accession: NZ_CP028893
• Organism: Vibrio cholerae strain Sa5Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A271VGY0
• Locus tag: Sa5Y_RS15875
• Protein ID: WP_000869987.1
• Coordinates: 368167..368454 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: W9USL4
• Locus tag: Sa5Y_RS15870
• Protein ID: WP_000086650.1
• Coordinates: 367889..368170 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Sa5Y_RS15820	363042..363134	+	93	WP_108243993.1	DUF3265 domain-containing protein	-
Sa5Y_RS15830	363318..363500	+	183	WP_108243994.1	DUF645 family protein	-
Sa5Y_RS15835	363582..364541	-	960	WP_094393086.1	IS30 family transposase	-
Sa5Y_RS15845	364758..365246	+	489	WP_108243996.1	dual specificity protein phosphatase family protein	-
Sa5Y_RS15850	365374..365994	+	621	WP_108243997.1	HNH endonuclease	-
Sa5Y_RS15855	365957..366109	+	153	WP_079881747.1	DUF3265 domain-containing protein	-
Sa5Y_RS15860	366137..366613	+	477	WP_069217461.1	GNAT family N-acetyltransferase	-
Sa5Y_RS15865	366759..367688	+	930	WP_108243998.1	hypothetical protein	-
Sa5Y_RS15870	367889..368170	+	282	WP_000086650.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Sa5Y_RS15875	368167..368454	+	288	WP_000869987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Sa5Y_RS15880	368614..368892	+	279	WP_059261376.1	hypothetical protein	-
Sa5Y_RS15885	368889..369239	+	351	WP_108243999.1	hypothetical protein	-
Sa5Y_RS15895	369384..369899	+	516	WP_108244112.1	GNAT family N-acetyltransferase	-
Sa5Y_RS15900	369922..370881	-	960	WP_094393086.1	IS30 family transposase	-
Sa5Y_RS15905	371116..371631	+	516	WP_001894921.1	lipocalin family protein	-
Sa5Y_RS15910	371788..372561	+	774	WP_114762515.1	hypothetical protein	-
Sa5Y_RS15920	372919..373155	+	237	WP_108243964.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306104..472928	166824
	-	inside	Integron	-	-	307268..474111	166843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11031.76 Da
• Isoelectric Point: 6.0710

>T104322 WP_000869987.1 NZ_CP028893:368167-368454 [Vibrio cholerae]
MKVVWSPLALQKLGDAAEFIAFDNPSAAEKWVNEVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQLLTEHDV

Nucleotide

• Length: 288 bp

>T104322 NZ_CP028893:368167-368454 [Vibrio cholerae]
ATGAAAGTAGTTTGGTCACCTCTAGCGTTACAAAAACTGGGTGATGCCGCAGAGTTTATTGCTTTTGATAACCCATCAGC
TGCAGAAAAGTGGGTGAATGAAGTATTCGACAAAACGGAATTGCTCGGCTCAATGCCAGAAATGGGCCGCATGGTTCCTG
AAATGCCTCATACGAACTACCGTGAAATAATTTTTGGTCATTACCGCATTATTTATAGTTTGAGCCACGAAATCCGCGTT
CTAACAGTTCGTAACTGTCGTCAATTGCTCACGGAACATGATGTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10347.90 Da
• Isoelectric Point: 5.1548

>AT104322 WP_000086650.1 NZ_CP028893:367889-368170 [Vibrio cholerae]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGVSN
EDARSQVLGRIIK

Nucleotide

• Length: 282 bp

>AT104322 NZ_CP028893:367889-368170 [Vibrio cholerae]
ATGAGCCGTATTCACCTTGATCAAGATATTCAGCCTTTGTCTGAGTTCCGTGCTGGCGTTGCATCATTTATCAAACAGAT
CAATGAAACTCGTCGACCATTAGTTATTACACAACGAGGTAAAGGTGTAGCCGTTGTTCTTGACGTTGCGGAATATGAAG
CAATGCAAGAGAAAATCGAATTACTTGAAGAAATGCGTACTGCAGAAGCTCAATTGGCTGCAGGTTTGGGTGTATCAAAC
GAAGATGCTCGTTCACAAGTTCTGGGACGCATCATCAAATGA

Structures

Toxin

• AlphaFold DB: A0A271VGY0

Antitoxin

• AlphaFold DB: W9USL4