Detailed information of TA system TA104320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 309952..310517
• Replicon: chromosome
• Accession: NZ_CP028893
• Organism: Vibrio cholerae strain Sa5Y

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Sa5Y_RS15240
• Protein ID: WP_162298932.1
• Coordinates: 310230..310517 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D0IIK3
• Locus tag: Sa5Y_RS15235
• Protein ID: WP_000086649.1
• Coordinates: 309952..310233 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Sa5Y_RS15200	305942..306493	+	552	WP_001894072.1	translation initiation factor IF-3	-
Sa5Y_RS15205	306597..306791	+	195	WP_001885060.1	50S ribosomal protein L35	-
Sa5Y_RS15210	306833..307186	+	354	WP_001138366.1	50S ribosomal protein L20	-
Sa5Y_RS15215	307268..308230	-	963	WP_094392037.1	integron integrase IntIA	-
Sa5Y_RS15220	308467..309102	+	636	WP_076008571.1	hypothetical protein	-
Sa5Y_RS15230	309236..309751	+	516	WP_000090012.1	hypothetical protein	-
Sa5Y_RS15235	309952..310233	+	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
Sa5Y_RS15240	310230..310517	+	288	WP_162298932.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Sa5Y_RS15245	310760..311179	+	420	WP_108244106.1	GNAT family N-acetyltransferase	-
Sa5Y_RS15250	311324..311821	+	498	WP_033933253.1	GNAT family N-acetyltransferase	-
Sa5Y_RS15255	311958..312578	+	621	WP_108243959.1	hypothetical protein	-
Sa5Y_RS15260	312722..313216	+	495	WP_000573468.1	GNAT family N-acetyltransferase	-
Sa5Y_RS15265	313378..313914	+	537	WP_000831688.1	hypothetical protein	-
Sa5Y_RS15280	314457..314570	+	114	WP_075042603.1	acetyltransferase	-
Sa5Y_RS15285	314519..314800	+	282	WP_002023460.1	DUF1289 domain-containing protein	-
Sa5Y_RS15300	315082..315366	+	285	WP_000048762.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	306104..472928	166824
	-	inside	Integron	-	-	307268..474111	166843

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.74 Da
• Isoelectric Point: 6.0710

>T104320 WP_162298932.1 NZ_CP028893:310230-310517 [Vibrio cholerae]
MKVVWSPLALQKLGDAAEFIALDNPSAAEKWVNEVFDKTELLGSMPEMGRMVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQLITEHDV

Nucleotide

• Length: 288 bp

>T104320 NZ_CP028893:310230-310517 [Vibrio cholerae]
ATGAAAGTAGTTTGGTCACCTCTAGCGTTACAAAAACTGGGTGATGCCGCAGAGTTTATTGCTTTGGATAACCCATCAGC
TGCAGAAAAGTGGGTGAATGAAGTATTCGACAAAACGGAATTGCTCGGCTCAATGCCAGAAATGGGCCGCATGGTTCCTG
AAATGCCTCATACGAACTACCGTGAAATAATTTTTGGTCATTACCGCATTATTTATAGTTTGAGCCACGAAATCCGCGTT
CTAACAGTTCGTAACTGTCGTCAATTGATCACGGAACATGATGTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10361.93 Da
• Isoelectric Point: 5.1548

>AT104320 WP_000086649.1 NZ_CP028893:309952-310233 [Vibrio cholerae]
MSRIHLDQDIQPLSEFRAGVASFIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLAAGLGISN
EDARSQVLGRIIK

Nucleotide

• Length: 282 bp

>AT104320 NZ_CP028893:309952-310233 [Vibrio cholerae]
ATGAGCCGTATTCACCTTGATCAAGATATTCAGCCTTTGTCTGAGTTTCGTGCTGGCGTTGCATCATTTATCAAACAGAT
CAATGAGACTCGTCGACCATTGGTTATTACACAACGAGGTAAAGGTGTAGCCGTTGTTCTTGACGTTGCGGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTCGAAGAAATGCGTACTGCAGAAGCTCAATTGGCTGCAGGTTTAGGTATATCAAAC
GAAGATGCTCGTTCACAAGTTCTGGGGCGCATCATCAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0K9UIW4