Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 27274..27866 Replicon plasmid p6_3214
Accession NZ_CP028856
Organism Klebsiella pneumoniae strain 3214

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag DBX64_RS30300 Protein ID WP_000520549.1
Coordinates 27618..27866 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag DBX64_RS30295 Protein ID WP_000681613.1
Coordinates 27274..27621 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBX64_RS30265 23275..23547 - 273 WP_001328551.1 TrbC/VirB2 family protein -
DBX64_RS30270 23615..24235 - 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
DBX64_RS30275 24592..24807 - 216 WP_000760375.1 EexN family lipoprotein -
DBX64_RS30280 24804..25097 - 294 WP_060578246.1 LuxR family transcriptional regulator -
DBX64_RS30285 25174..26403 - 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
DBX64_RS30290 26407..26910 - 504 WP_169023835.1 hypothetical protein -
DBX64_RS30295 27274..27621 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DBX64_RS30300 27618..27866 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
DBX64_RS30305 28173..28478 - 306 WP_072182957.1 hypothetical protein -
DBX64_RS30310 28546..28818 - 273 WP_001272373.1 hypothetical protein -
DBX64_RS30315 29267..29494 - 228 WP_000801440.1 hypothetical protein -
DBX64_RS30320 29481..29732 - 252 WP_001230707.1 hypothetical protein -
DBX64_RS30325 29729..30271 - 543 WP_001083906.1 hypothetical protein -
DBX64_RS30330 30376..30891 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31145 31145


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T104318 WP_000520549.1 NZ_CP028856:c27866-27618 [Klebsiella pneumoniae]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T104318 NZ_CP028856:c27866-27618 [Klebsiella pneumoniae]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT104318 WP_000681613.1 NZ_CP028856:c27621-27274 [Klebsiella pneumoniae]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT104318 NZ_CP028856:c27621-27274 [Klebsiella pneumoniae]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGGAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References