Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 32473..33020 Replicon plasmid pCTX_3214
Accession NZ_CP028854
Organism Klebsiella pneumoniae strain 3214

Toxin (Protein)


Gene name parE Uniprot ID A0A4Y1YQH8
Locus tag DBX64_RS29525 Protein ID WP_012881118.1
Coordinates 32739..33020 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E2GHF7
Locus tag DBX64_RS29520 Protein ID WP_012881119.1
Coordinates 32473..32742 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBX64_RS29485 28102..28314 + 213 WP_001309245.1 ANR family transcriptional regulator -
DBX64_RS29490 28559..29020 + 462 WP_001233838.1 thermonuclease family protein -
DBX64_RS29495 29066..29275 + 210 WP_001298565.1 hemolysin expression modulator Hha -
DBX64_RS29500 29313..29903 + 591 WP_000766807.1 DUF2726 domain-containing protein -
DBX64_RS29505 30143..30397 + 255 WP_000083830.1 replication regulatory protein RepA -
DBX64_RS29510 30635..30709 + 75 WP_001365705.1 RepA leader peptide Tap -
DBX64_RS29515 30702..31559 + 858 WP_000130646.1 incFII family plasmid replication initiator RepA -
DBX64_RS29520 32473..32742 + 270 WP_012881119.1 ABC transporter ATPase Antitoxin
DBX64_RS29525 32739..33020 + 282 WP_012881118.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DBX64_RS29530 33066..33914 + 849 WP_012881117.1 3'-5' exonuclease -
DBX64_RS29535 34031..34513 + 483 WP_001311056.1 hypothetical protein -
DBX64_RS29540 34548..35654 - 1107 Protein_39 maltoporin LamB -
DBX64_RS29545 36106..36981 - 876 WP_001617865.1 class A extended-spectrum beta-lactamase CTX-M-14 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-14 - 1..70299 70299
- flank IS/Tn blaCTX-M-14 - 36106..38493 2387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10875.53 Da        Isoelectric Point: 8.4615

>T104317 WP_012881118.1 NZ_CP028854:32739-33020 [Klebsiella pneumoniae]
MMEIFWTILASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T104317 NZ_CP028854:32739-33020 [Klebsiella pneumoniae]
ATGATGGAGATATTCTGGACCATACTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTATATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10206.54 Da        Isoelectric Point: 8.8735

>AT104317 WP_012881119.1 NZ_CP028854:32473-32742 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSATAWRD
EISRKVAGK

Download         Length: 270 bp

>AT104317 NZ_CP028854:32473-32742 [Klebsiella pneumoniae]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACTTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGATATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATAAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Y1YQH8

Antitoxin

Source ID Structure
AlphaFold DB A0A4Y1YSD8

References