Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 54261..54786 Replicon plasmid p3_3214
Accession NZ_CP028853
Organism Klebsiella pneumoniae strain 3214

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag DBX64_RS29060 Protein ID WP_013023785.1
Coordinates 54481..54786 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag DBX64_RS29055 Protein ID WP_001568025.1
Coordinates 54261..54479 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBX64_RS29030 49417..50196 + 780 WP_013023780.1 hypothetical protein -
DBX64_RS29035 50403..51995 + 1593 WP_015344964.1 hypothetical protein -
DBX64_RS29040 52029..53156 + 1128 WP_013023782.1 DUF4238 domain-containing protein -
DBX64_RS29045 53153..53443 + 291 WP_013023783.1 hypothetical protein -
DBX64_RS29050 53433..53630 - 198 WP_017899886.1 hypothetical protein -
DBX64_RS29055 54261..54479 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DBX64_RS29060 54481..54786 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
DBX64_RS29065 54955..55350 + 396 WP_017899885.1 hypothetical protein -
DBX64_RS29070 55377..55691 + 315 WP_053389906.1 hypothetical protein -
DBX64_RS29075 55702..56718 + 1017 WP_017899884.1 hypothetical protein -
DBX64_RS29080 56916..57710 + 795 WP_004197635.1 site-specific integrase -
DBX64_RS29085 58182..58361 - 180 WP_006788217.1 hypothetical protein -
DBX64_RS29090 58481..59107 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrS1 / blaLAP-2 / tet(A) / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB91 / mph(A) / aph(3')-Ia - 1..94396 94396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T104316 WP_013023785.1 NZ_CP028853:54481-54786 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T104316 NZ_CP028853:54481-54786 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT104316 WP_001568025.1 NZ_CP028853:54261-54479 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT104316 NZ_CP028853:54261-54479 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References