Detailed information of TA system TA104313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67834..68504
• Replicon: plasmid pVIR_3214
• Accession: NZ_CP028852
• Organism: Klebsiella pneumoniae strain 3214

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DBX64_RS27830
• Protein ID: WP_004213072.1
• Coordinates: 68061..68504 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DBX64_RS27825
• Protein ID: WP_004213073.1
• Coordinates: 67834..68064 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBX64_RS27800	64057..64956	-	900	WP_004225022.1	nucleotide-binding protein	-
DBX64_RS27805	64946..65236	-	291	WP_004213078.1	hypothetical protein	-
DBX64_RS27810	65588..65794	+	207	WP_004213077.1	hypothetical protein	-
DBX64_RS27815	65784..66077	-	294	WP_004213076.1	hypothetical protein	-
DBX64_RS27820	66093..67226	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DBX64_RS27825	67834..68064	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DBX64_RS27830	68061..68504	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DBX64_RS27835	68518..68934	-	417	Protein_72	restriction endonuclease	-
DBX64_RS27840	68938..69231	-	294	Protein_73	transposase	-
DBX64_RS27845	69648..70288	+	641	Protein_74	response regulator	-
DBX64_RS27850	70803..71209	-	407	Protein_75	GAF domain-containing protein	-
DBX64_RS27855	71300..72220	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
DBX64_RS27860	72269..72760	-	492	WP_011251327.1	DM13 domain-containing protein	-
DBX64_RS27865	72823..73098	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..231431	231431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T104313 WP_004213072.1 NZ_CP028852:68061-68504 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T104313 NZ_CP028852:68061-68504 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT104313 WP_004213073.1 NZ_CP028852:67834-68064 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT104313 NZ_CP028852:67834-68064 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620