Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 23822..24344 Replicon plasmid pNDM_3214
Accession NZ_CP028851
Organism Klebsiella pneumoniae strain 3214

Toxin (Protein)


Gene name stbE Uniprot ID A0A7H0EVH6
Locus tag DBX64_RS26320 Protein ID WP_014839901.1
Coordinates 24060..24344 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A7H0EVH5
Locus tag DBX64_RS26315 Protein ID WP_014839900.1
Coordinates 23822..24070 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBX64_RS26290 19000..19356 + 357 WP_017901071.1 hypothetical protein -
DBX64_RS26295 19730..20128 + 399 WP_014839899.1 H-NS histone family protein -
DBX64_RS26300 20265..20873 + 609 WP_024266306.1 DUF2913 family protein -
DBX64_RS26305 21417..22049 + 633 WP_001567369.1 hypothetical protein -
DBX64_RS26310 22078..23481 - 1404 WP_057061543.1 ISNCY-like element ISKpn21 family transposase -
DBX64_RS26315 23822..24070 + 249 WP_014839900.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
DBX64_RS26320 24060..24344 + 285 WP_014839901.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DBX64_RS26325 24535..25011 + 477 WP_042934526.1 hypothetical protein -
DBX64_RS26330 25017..25694 + 678 WP_042934501.1 hypothetical protein -
DBX64_RS26335 25711..26430 - 720 WP_042934502.1 cation:proton antiporter -
DBX64_RS26340 26671..27273 - 603 WP_014839902.1 hypothetical protein -
DBX64_RS26345 27270..27791 - 522 WP_048336588.1 hypothetical protein -
DBX64_RS26350 27872..28816 - 945 WP_014839903.1 DsbA family protein -
DBX64_RS26355 28879..29088 - 210 WP_125316032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / blaNDM-1 - 1..238037 238037
- flank IS/Tn - - 22078..23481 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10866.46 Da        Isoelectric Point: 10.5831

>T104312 WP_014839901.1 NZ_CP028851:24060-24344 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Download         Length: 285 bp

>T104312 NZ_CP028851:24060-24344 [Klebsiella pneumoniae]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTACAACGCAACCCGACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9071.29 Da        Isoelectric Point: 3.8832

>AT104312 WP_014839900.1 NZ_CP028851:23822-24070 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Download         Length: 249 bp

>AT104312 NZ_CP028851:23822-24070 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H0EVH6

Antitoxin

Source ID Structure
AlphaFold DB A0A7H0EVH5

References