Detailed information of TA system TA104294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5191594..5192180
• Replicon: chromosome
• Accession: NZ_CP028849
• Organism: Pseudomonas aeruginosa strain IMP68

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: DBZ62_RS24785
• Protein ID: WP_003120987.1
• Coordinates: 5191881..5192180 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DBZ62_RS24780
• Protein ID: WP_003448662.1
• Coordinates: 5191594..5191884 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBZ62_RS24760	5186746..5186955	+	210	WP_003105733.1	cold-shock protein	-
DBZ62_RS24765	5187176..5189065	+	1890	WP_003105732.1	hypothetical protein	-
DBZ62_RS24770	5189062..5191038	+	1977	WP_017002277.1	DEAD/DEAH box helicase	-
DBZ62_RS24775	5191179..5191523	+	345	WP_003448665.1	hypothetical protein	-
DBZ62_RS24780	5191594..5191884	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
DBZ62_RS24785	5191881..5192180	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBZ62_RS24790	5192382..5193503	+	1122	WP_017002276.1	TcpQ domain-containing protein	-
DBZ62_RS24795	5193503..5195212	+	1710	WP_020751093.1	PilN family type IVB pilus formation outer membrane protein	-
DBZ62_RS24800	5195216..5196541	+	1326	WP_017002275.1	type 4b pilus protein PilO2	-
DBZ62_RS24805	5196531..5197064	+	534	WP_017002274.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5164090..5276164	112074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T104294 WP_003120987.1 NZ_CP028849:c5192180-5191881 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T104294 NZ_CP028849:c5192180-5191881 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT104294 WP_003448662.1 NZ_CP028849:c5191884-5191594 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT104294 NZ_CP028849:c5191884-5191594 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAGTCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTAA

Structures

Toxin

• AlphaFold DB: G8CP73