Detailed information of TA system TA104280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 32603..33137
• Replicon: plasmid unnamed3
• Accession: NZ_CP028838
• Organism: Enterococcus faecalis strain FC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: C9432_RS16160
• Protein ID: WP_002360769.1
• Coordinates: 32862..33137 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: C9432_RS16155
• Protein ID: WP_126264777.1
• Coordinates: 32603..32869 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9432_RS16120	28686..29468	+	783	WP_010785116.1	replication-relaxation family protein	-
C9432_RS16125	30096..30362	+	267	WP_002365974.1	hypothetical protein	-
C9432_RS16130	30395..30895	+	501	WP_002360775.1	DnaJ domain-containing protein	-
C9432_RS16135	30908..31155	+	248	Protein_29	DUF3850 domain-containing protein	-
C9432_RS16140	31231..31647	+	417	WP_002368093.1	single-stranded DNA-binding protein	-
C9432_RS16145	31671..32255	+	585	WP_002400616.1	thermonuclease family protein	-
C9432_RS16150	32252..32500	+	249	WP_010830037.1	hypothetical protein	-
C9432_RS16155	32603..32869	+	267	WP_126264777.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C9432_RS16160	32862..33137	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
C9432_RS16170	33940..34125	+	186	WP_002365983.1	hypothetical protein	-
C9432_RS16175	34110..34301	+	192	WP_002399282.1	DpnD/PcfM family protein	-
C9432_RS16180	34325..34915	+	591	WP_126264776.1	hypothetical protein	-
C9432_RS16185	35020..35799	+	780	WP_126264775.1	hypothetical protein	-
C9432_RS16190	35799..37142	+	1344	WP_126264774.1	PcfJ domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	prgB/asc10 / prgB/asc10 / EF0485	1..49583	49583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T104280 WP_002360769.1 NZ_CP028838:32862-33137 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T104280 NZ_CP028838:32862-33137 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGATTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9786.12 Da
• Isoelectric Point: 4.4791

>AT104280 WP_126264777.1 NZ_CP028838:32603-32869 [Enterococcus faecalis]
MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFIINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT104280 NZ_CP028838:32603-32869 [Enterococcus faecalis]
TTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACTATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATAATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAACGTTCATGGTGGATTCTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7