Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 45418..46555 Replicon plasmid unnamed1
Accession NZ_CP028836
Organism Enterococcus faecalis strain FC

Toxin (Protein)


Gene name zeta Uniprot ID P0A4M2
Locus tag C9432_RS15275 Protein ID WP_002332783.1
Coordinates 45418..46281 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag C9432_RS15280 Protein ID WP_002326825.1
Coordinates 46283..46555 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C9432_RS15230 40531..41439 - 909 WP_001255866.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
C9432_RS15235 41458..41598 - 141 Protein_45 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
C9432_RS15245 41744..42481 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
C9432_RS15250 42606..42689 - 84 WP_032501549.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
C9432_RS15260 43144..43824 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
C9432_RS15265 43858..44364 - 507 WP_002415429.1 trimethoprim-resistant dihydrofolate reductase DfrG -
C9432_RS15270 44661..44978 - 318 WP_002326830.1 hypothetical protein -
C9432_RS15275 45418..46281 - 864 WP_002332783.1 toxin zeta Toxin
C9432_RS15280 46283..46555 - 273 WP_002326825.1 antitoxin Antitoxin
C9432_RS15285 46573..46782 - 210 WP_000527318.1 peptide-binding protein -
C9432_RS15290 46880..47776 - 897 WP_002326827.1 ParA family protein -
C9432_RS15295 47879..48139 - 261 Protein_55 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
C9432_RS15305 48309..49046 - 738 WP_001038795.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
C9432_RS15310 49171..49254 - 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
C9432_RS15320 49686..50057 - 372 WP_002358205.1 hypothetical protein -
C9432_RS15325 50050..50895 - 846 WP_000239313.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / aph(3')-III / ant(6)-Ia / dfrG / cat / tet(L) / tet(M) - 1..90170 90170
- inside IS/Tn erm(B) / aph(3')-III / ant(6)-Ia / dfrG - 37618..49046 11428


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(28-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32675.27 Da        Isoelectric Point: 7.3939

>T104275 WP_002332783.1 NZ_CP028836:c46281-45418 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T104275 NZ_CP028836:c46281-45418 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT104275 WP_002326825.1 NZ_CP028836:c46555-46283 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT104275 NZ_CP028836:c46555-46283 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P0A4M2

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References