Detailed information of TA system TA104274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 31011..31582
• Replicon: plasmid unnamed1
• Accession: NZ_CP028836
• Organism: Enterococcus faecalis strain FC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S4H3R9
• Locus tag: C9432_RS15130
• Protein ID: WP_010784114.1
• Coordinates: 31011..31352 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: C9432_RS15135
• Protein ID: WP_002362431.1
• Coordinates: 31352..31582 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9432_RS15110	26410..27693	-	1284	WP_002405123.1	hypothetical protein	-
C9432_RS15120	29094..29696	-	603	WP_002362434.1	Fic family protein	-
C9432_RS15125	29961..30899	-	939	WP_002362433.1	hypothetical protein	-
C9432_RS15130	31011..31352	-	342	WP_010784114.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C9432_RS15135	31352..31582	-	231	WP_002362431.1	hypothetical protein	Antitoxin
C9432_RS15140	31786..32406	+	621	WP_002367784.1	recombinase family protein	-
C9432_RS15145	32396..32710	+	315	WP_002367785.1	hypothetical protein	-
C9432_RS15155	33070..33264	+	195	WP_002367787.1	hypothetical protein	-
C9432_RS15160	33276..33467	+	192	WP_002367788.1	hypothetical protein	-
C9432_RS15165	33637..33852	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
C9432_RS15170	33853..34194	+	342	WP_002367792.1	bacteriocin immunity protein	-
C9432_RS15175	34610..35128	+	519	WP_002367793.1	hypothetical protein	-
C9432_RS15185	35383..35469	+	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
C9432_RS15190	35726..36022	+	297	WP_002367795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aph(3')-III / ant(6)-Ia / dfrG / cat / tet(L) / tet(M)	-	1..90170	90170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13270.47 Da
• Isoelectric Point: 7.9750

>T104274 WP_010784114.1 NZ_CP028836:c31352-31011 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEYLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T104274 NZ_CP028836:c31352-31011 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT104274 WP_002362431.1 NZ_CP028836:c31582-31352 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT104274 NZ_CP028836:c31582-31352 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: S4H3R9

Antitoxin

• AlphaFold DB: R3KHK9