Detailed information of TA system TA104269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2876275..2876846
• Replicon: chromosome
• Accession: NZ_CP028835
• Organism: Enterococcus faecalis strain FC

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: C9432_RS14700
• Protein ID: WP_002354774.1
• Coordinates: 2876275..2876616 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: C9432_RS14705
• Protein ID: WP_002354773.1
• Coordinates: 2876616..2876846 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9432_RS14690	2872290..2875904	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
C9432_RS14700	2876275..2876616	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
C9432_RS14705	2876616..2876846	-	231	WP_002354773.1	hypothetical protein	Antitoxin
C9432_RS14710	2877260..2877475	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
C9432_RS14715	2877614..2878606	+	993	WP_002365428.1	biotin--[acetyl-CoA-carboxylase] ligase	-
C9432_RS14720	2878673..2879305	-	633	WP_002358972.1	RloB family protein	-
C9432_RS14725	2879314..2880609	-	1296	WP_010815865.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T104269 WP_002354774.1 NZ_CP028835:c2876616-2876275 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T104269 NZ_CP028835:c2876616-2876275 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT104269 WP_002354773.1 NZ_CP028835:c2876846-2876616 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT104269 NZ_CP028835:c2876846-2876616 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCGGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812