Detailed information of TA system TA104268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 496210..496744
• Replicon: chromosome
• Accession: NZ_CP028835
• Organism: Enterococcus faecalis strain FC

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: C9432_RS02665
• Protein ID: WP_002360769.1
• Coordinates: 496469..496744 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: C9432_RS02660
• Protein ID: WP_002369771.1
• Coordinates: 496210..496476 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9432_RS02625	492068..492853	+	786	WP_002360778.1	replication-relaxation family protein	-
C9432_RS02630	492859..493347	+	489	WP_010710133.1	hypothetical protein	-
C9432_RS02635	493939..494205	+	267	WP_002377947.1	hypothetical protein	-
C9432_RS02640	494238..494738	+	501	WP_002360775.1	DnaJ domain-containing protein	-
C9432_RS02645	494751..494999	+	249	WP_002360773.1	DUF3850 domain-containing protein	-
C9432_RS02650	495075..495491	+	417	WP_010710134.1	single-stranded DNA-binding protein	-
C9432_RS02655	495515..496099	+	585	WP_002377949.1	thermonuclease family protein	-
C9432_RS02660	496210..496476	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
C9432_RS02665	496469..496744	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
C9432_RS02670	496935..497615	-	681	Protein_464	IS6-like element IS1216 family transposase	-
C9432_RS02675	497803..498777	+	975	WP_002355428.1	choloylglycine hydrolase	-
C9432_RS02685	499446..500054	-	609	Protein_466	IS6 family transposase	-
C9432_RS02690	500086..500271	-	186	WP_002358660.1	hypothetical protein	-
C9432_RS02695	500301..500630	+	330	WP_002358661.1	LPXTG cell wall anchor domain-containing protein	-
C9432_RS02700	500758..501696	+	939	WP_002370935.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	EF0485 / bsh / cylR2 / cylR1 / cylL / cylS / cylM / cylB / cylA / cylI / esp	470835..577792	106957

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T104268 WP_002360769.1 NZ_CP028835:496469-496744 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T104268 NZ_CP028835:496469-496744 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT104268 WP_002369771.1 NZ_CP028835:496210-496476 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT104268 NZ_CP028835:496210-496476 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7