Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/upstrm_HI1419-dnstrm_HI1420
Location 707351..707947 Replicon chromosome
Accession NZ_CP028826
Organism Pseudomonas fluorescens strain MS82

Toxin (Protein)


Gene name relE Uniprot ID -
Locus tag DBV33_RS03155 Protein ID WP_085611238.1
Coordinates 707645..707947 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag DBV33_RS03150 Protein ID WP_085609233.1
Coordinates 707351..707641 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBV33_RS03130 703651..705771 - 2121 WP_108559384.1 TonB-dependent copper receptor -
DBV33_RS03135 705856..706302 - 447 WP_108559386.1 DUF2946 domain-containing protein -
DBV33_RS03140 706324..706803 - 480 WP_074689676.1 copper chaperone PCu(A)C -
DBV33_RS03145 706852..707253 - 402 WP_108559388.1 DUF2946 domain-containing protein -
DBV33_RS03150 707351..707641 - 291 WP_085609233.1 putative addiction module antidote protein Antitoxin
DBV33_RS03155 707645..707947 - 303 WP_085611238.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DBV33_RS03160 708139..708867 - 729 WP_108559390.1 cobalt-precorrin-6A reductase -
DBV33_RS03165 708864..709961 - 1098 WP_108559392.1 cobalt-precorrin-5B (C(1))-methyltransferase -
DBV33_RS03170 709954..711165 - 1212 WP_108559394.1 precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE -
DBV33_RS03175 711387..712058 + 672 WP_064379185.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-91)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11408.07 Da        Isoelectric Point: 9.4236

>T104240 WP_085611238.1 NZ_CP028826:c707947-707645 [Pseudomonas fluorescens]
MIEFERSPAFADWLDSMKDTNGKARIIARIRGAEHGNMGDCEFVGSAVYEMRVHYGPGYRMYFTRKGNVIYLLLLGGDKS
TQKRDIKRAIRMAQSIGNEE

Download         Length: 303 bp

>T104240 NZ_CP028826:c707947-707645 [Pseudomonas fluorescens]
ATGATCGAATTTGAACGATCCCCTGCATTTGCCGATTGGCTCGACTCGATGAAAGACACCAACGGCAAAGCTCGCATCAT
CGCCAGAATTCGAGGTGCAGAGCATGGAAATATGGGTGATTGCGAGTTCGTTGGCAGTGCCGTTTATGAAATGCGCGTTC
ATTACGGTCCCGGCTACAGGATGTACTTCACACGTAAAGGTAACGTGATTTATCTGCTACTGCTGGGGGGCGACAAGTCG
ACACAAAAACGAGATATCAAACGCGCCATCCGGATGGCGCAAAGCATCGGTAATGAGGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10342.84 Da        Isoelectric Point: 4.7961

>AT104240 WP_085609233.1 NZ_CP028826:c707641-707351 [Pseudomonas fluorescens]
MTEKFTRFDAAEFLKTPEEMAAYLDACFDEDAGDGVLIRAALNDIARAQGMTQVARDAGLGRESLYKALSSTGNPEFATI
MKVMKALGLRLHAVAG

Download         Length: 291 bp

>AT104240 NZ_CP028826:c707641-707351 [Pseudomonas fluorescens]
ATGACCGAAAAATTCACCCGCTTCGACGCTGCAGAGTTTCTTAAAACGCCGGAGGAAATGGCGGCTTACCTGGACGCCTG
TTTTGACGAAGATGCTGGCGATGGCGTTCTCATCCGTGCAGCGCTTAATGACATTGCCCGCGCCCAAGGCATGACTCAGG
TTGCGCGGGATGCAGGTCTGGGTCGTGAAAGCCTTTATAAAGCGCTGAGCAGCACTGGAAATCCGGAATTCGCGACTATC
ATGAAAGTCATGAAGGCGCTGGGTTTGCGGCTGCATGCCGTAGCAGGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References