Detailed information of TA system TA104236

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2264210..2264873
• Replicon: chromosome
• Accession: NZ_CP028813
• Organism: Edwardsiella ictaluri strain MS-17-156

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: C5BAS3
• Locus tag: DBV23_RS10790
• Protein ID: WP_015872556.1
• Coordinates: 2264457..2264873 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: C5BAS4
• Locus tag: DBV23_RS10785
• Protein ID: WP_015872557.1
• Coordinates: 2264210..2264476 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBV23_RS10765	2259709..2260359	-	651	WP_035609190.1	hemolysin III family protein	-
DBV23_RS10770	2260968..2261840	+	873	WP_015872560.1	nucleoside-specific channel-forming protein Tsx	-
DBV23_RS10775	2262002..2262826	+	825	WP_015872559.1	shikimate 5-dehydrogenase	-
DBV23_RS10780	2262867..2263862	-	996	WP_015872558.1	tRNA-modifying protein YgfZ	-
DBV23_RS10785	2264210..2264476	+	267	WP_015872557.1	FAD assembly factor SdhE	Antitoxin
DBV23_RS10790	2264457..2264873	+	417	WP_015872556.1	protein YgfX	Toxin
DBV23_RS10795	2264898..2265410	-	513	WP_015872555.1	flavodoxin FldB	-
DBV23_RS10800	2265568..2266467	+	900	WP_015872554.1	site-specific tyrosine recombinase XerD	-
DBV23_RS10805	2266495..2267211	+	717	WP_015872553.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
DBV23_RS10810	2267216..2268949	+	1734	WP_015872552.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (2-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 16145.89 Da
• Isoelectric Point: 11.3233

>T104236 WP_015872556.1 NZ_CP028813:2264457-2264873 [Edwardsiella ictaluri]
VALWRCNVHASWRTQVMFLLGHGALVLAILLAPWPENYSVFWLILLVAVIFECIRSQRLIARNSGELQRLAPQVWHWQLR
EWRLARRPWVSDLGALLILQSTHGTPCRRRLWLAADSMSREEWGQLRRALLDTGDDRV

Nucleotide

• Length: 417 bp

>T104236 NZ_CP028813:2264457-2264873 [Edwardsiella ictaluri]
GTGGCCCTTTGGCGCTGTAATGTTCACGCCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCATGGCGCGCTGGTATT
GGCGATCCTGTTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTTGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGTCTGATTGCGCGTAACAGCGGTGAGCTACAGCGGCTGGCCCCACAGGTATGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGGCGCCCCTGGGTTAGCGATCTGGGAGCGTTGCTGATCCTGCAGAGTACCCATGGCACGCCGTG
CCGCCGTCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCGCGCTGCTCGATACGG
GCGACGATCGCGTATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10364.92 Da
• Isoelectric Point: 5.6951

>AT104236 WP_015872557.1 NZ_CP028813:2264210-2264476 [Edwardsiella ictaluri]
MDINNKARVHWACRRGMRELDIAIMPFFEHEYDALNDADKQTFVRLLSCDDPELFKWLMNQGKPDDTDLARMITLIQTRN
KERGPLAL

Nucleotide

• Length: 267 bp

>AT104236 NZ_CP028813:2264210-2264476 [Edwardsiella ictaluri]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGTGAGCTGGATATCGCCATTATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGACTGCTGTCCTGTGACGATCCTGAAC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGACGATACCGATCTGGCCCGTATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGCCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: C5BAS3

Antitoxin

• AlphaFold DB: C5BAS4