Detailed information of TA system TA104232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 134321..134970
• Replicon: plasmid p875
• Accession: NZ_CP028810
• Organism: Burkholderia contaminans strain SK875

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SK875_RS39470
• Protein ID: WP_027813527.1
• Coordinates: 134560..134970 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6P2T8K5
• Locus tag: SK875_RS39465
• Protein ID: WP_027813526.1
• Coordinates: 134321..134563 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SK875_RS39445	129538..130395	-	858	WP_027813522.1	replication initiator protein A	-
SK875_RS39450	131138..132103	-	966	WP_027813523.1	ParB/RepB/Spo0J family partition protein	-
SK875_RS39455	132103..132774	-	672	WP_027813524.1	AAA family ATPase	-
SK875_RS39460	133177..134226	+	1050	WP_027813525.1	non-homologous end-joining DNA ligase	-
SK875_RS39465	134321..134563	+	243	WP_027813526.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SK875_RS39470	134560..134970	+	411	WP_027813527.1	type II toxin-antitoxin system VapC family toxin	Toxin
SK875_RS39475	135282..135629	+	348	WP_027813528.1	hypothetical protein	-
SK875_RS39480	135678..135878	+	201	WP_034176085.1	hypothetical protein	-
SK875_RS39485	136066..136719	+	654	WP_027813530.1	helix-turn-helix domain-containing protein	-
SK875_RS39490	136740..137045	+	306	WP_027813531.1	hypothetical protein	-
SK875_RS39495	137090..138175	+	1086	WP_027813532.1	hypothetical protein	-
SK875_RS39500	138186..138416	+	231	WP_027813533.1	hypothetical protein	-
SK875_RS39505	138426..138680	+	255	WP_046544062.1	hypothetical protein	-
SK875_RS39510	138896..139567	+	672	WP_034176084.1	hypothetical protein	-
SK875_RS39515	139649..139939	+	291	WP_027813562.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..200961	200961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14869.13 Da
• Isoelectric Point: 4.8604

>T104232 WP_027813527.1 NZ_CP028810:134560-134970 [Burkholderia contaminans]
MTLYLLDTNILSNVIRDPRGACATRIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIDANDMLIAAHALAVDAVLVTDNTAEFTRIAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T104232 NZ_CP028810:134560-134970 [Burkholderia contaminans]
ATGACGCTGTACCTGCTCGATACCAACATTTTGTCGAACGTGATCCGCGACCCGCGCGGCGCATGCGCCACCCGGATTGG
AGAAACGCCGCCCGAACAAGTGTGCACCTCGATCATCGTGGCCGCGGAATTGCGGTTCGGCGTCTGGAAGCGTGGCTCGT
CAACGCTCGCGCAGCGCGTCGAGCAGCTGCTCGCAAGCCTGACCGTGTTGCCGCTGCAACCGGACGCGGACCGCTGCTAC
GGGCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGTTGATCGACGCCAACGACATGCTGATCGCCGCGCATGCGCTGGC
CGTCGACGCCGTGCTGGTCACGGACAACACGGCTGAATTCACCCGTATCGCGGGGCTGCCGGTCGAAAACTGGCTGCGGC
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9375.64 Da
• Isoelectric Point: 4.6856

>AT104232 WP_027813526.1 NZ_CP028810:134321-134563 [Burkholderia contaminans]
MPNHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDEIFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT104232 NZ_CP028810:134321-134563 [Burkholderia contaminans]
ATGCCAAACCATGACACCCGCCACGCGCGCCTGTTCCGCAACGGGCGGAGCCAGGCCGTGCGGATCCCGCGCGAATTCGA
ACTGCCGGTGTCGGAGGTCACCATCCACCGGGAAGGTCGCCGCTTGATCATTGAGCCCGTAGAGGCCGCGCCCTTGCGCG
AGCTGTTCGCCCAATGGACCCCGCTGGACGAGATCTTCCCGGACATCGAGGACCTTCCGCCTGAACCGGTCGATATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A6P2T8K5