Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 59421..60015 Replicon plasmid p875
Accession NZ_CP028810
Organism Burkholderia contaminans strain SK875

Toxin (Protein)


Gene name PumA Uniprot ID A0A228JB64
Locus tag SK875_RS39100 Protein ID WP_027811055.1
Coordinates 59421..59714 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A228JAU5
Locus tag SK875_RS39105 Protein ID WP_027811056.1
Coordinates 59716..60015 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SK875_RS39075 54885..57014 + 2130 WP_039350079.1 ParB/RepB/Spo0J family partition protein -
SK875_RS39085 58058..58324 + 267 WP_027811052.1 hypothetical protein -
SK875_RS39090 58299..58850 + 552 WP_077186813.1 hypothetical protein -
SK875_RS39095 58895..59116 + 222 WP_027811054.1 DUF3717 domain-containing protein -
SK875_RS40280 59173..59316 + 144 WP_154233621.1 hypothetical protein -
SK875_RS39100 59421..59714 + 294 WP_027811055.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SK875_RS39105 59716..60015 + 300 WP_027811056.1 putative addiction module antidote protein Antitoxin
SK875_RS39110 60178..60489 - 312 WP_027811057.1 hypothetical protein -
SK875_RS39115 60737..61240 - 504 WP_143277545.1 hypothetical protein -
SK875_RS39120 62257..62658 + 402 WP_027811058.1 hypothetical protein -
SK875_RS39125 62651..64717 + 2067 WP_027811059.1 type IV secretion system DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..200961 200961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(11-87)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10854.50 Da        Isoelectric Point: 10.6086

>T104231 WP_027811055.1 NZ_CP028810:59421-59714 [Burkholderia contaminans]
MRIEQTDDFAKWLRGLRDHIARAQIAKRIQRLARGQYGDMKPVGAGVSEMRVHVGPGYRVYFVQRGSTLVVLLCGGDKST
QQRDIERAIALSAQLGD

Download         Length: 294 bp

>T104231 NZ_CP028810:59421-59714 [Burkholderia contaminans]
ATGAGAATCGAGCAGACAGACGACTTCGCGAAATGGTTGCGCGGCCTACGCGATCACATCGCGCGCGCGCAGATCGCCAA
GCGGATTCAACGGCTCGCGCGCGGCCAATATGGGGACATGAAGCCGGTGGGTGCCGGAGTGAGCGAAATGCGCGTGCACG
TCGGCCCCGGCTATCGCGTCTACTTTGTCCAACGAGGCTCAACGCTCGTCGTCCTGCTATGCGGTGGCGACAAGTCAACG
CAGCAGCGCGACATTGAGCGTGCTATCGCGCTTTCGGCGCAATTGGGAGATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10716.01 Da        Isoelectric Point: 4.6364

>AT104231 WP_027811056.1 NZ_CP028810:59716-60015 [Burkholderia contaminans]
MGIKTTPFDAAEYLDTPEAQAEYLRISFETGDSADIQEAIGTVARARGMTQIARDAGVGRESLYKALDEHGNPEFATILR
VVRALGLTLTVTTPVHEHA

Download         Length: 300 bp

>AT104231 NZ_CP028810:59716-60015 [Burkholderia contaminans]
ATGGGTATCAAGACAACGCCGTTCGATGCGGCCGAATACCTCGACACGCCCGAGGCGCAGGCCGAATATCTGCGGATTTC
GTTCGAGACCGGCGATTCCGCCGACATTCAGGAAGCCATCGGCACCGTGGCGCGTGCGCGTGGCATGACGCAGATTGCGC
GCGACGCGGGCGTCGGCCGCGAAAGCCTCTACAAAGCGCTGGACGAGCACGGCAACCCGGAATTCGCGACGATCCTGCGT
GTTGTGCGCGCGCTCGGCCTCACGTTGACCGTGACGACGCCCGTACACGAGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A228JB64

Antitoxin

Source ID Structure
AlphaFold DB A0A228JAU5

References