Detailed information of TA system TA104228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1851103..1851723
• Replicon: chromosome
• Accession: NZ_CP028808
• Organism: Burkholderia contaminans strain SK875

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: SK875_RS25670
• Protein ID: WP_095146199.1
• Coordinates: 1851103..1851420 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1J9PWC2
• Locus tag: SK875_RS25675
• Protein ID: WP_039344756.1
• Coordinates: 1851424..1851723 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SK875_RS25655	1847647..1849080	-	1434	WP_039344747.1	aldehyde dehydrogenase family protein	-
SK875_RS25660	1849110..1850750	-	1641	WP_039344750.1	acetolactate synthase large subunit	-
SK875_RS25670	1851103..1851420	+	318	WP_095146199.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SK875_RS25675	1851424..1851723	+	300	WP_039344756.1	putative addiction module antidote protein	Antitoxin
SK875_RS25680	1851772..1853058	-	1287	WP_039344759.1	DUF445 domain-containing protein	-
SK875_RS25685	1853229..1854449	-	1221	WP_039344761.1	FAD-dependent oxidoreductase	-
SK875_RS25690	1854439..1854765	-	327	WP_039344765.1	non-heme iron oxygenase ferredoxin subunit	-
SK875_RS25695	1854786..1855274	-	489	WP_011355917.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
SK875_RS25700	1855289..1856560	-	1272	WP_039344770.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-91)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11796.53 Da
• Isoelectric Point: 9.9432

>T104228 WP_095146199.1 NZ_CP028808:1851103-1851420 [Burkholderia contaminans]
MPYSAPTFSIRTTGVFDTWFAGLQDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRGTIWVILL
CGGDKSSQQADIRAAHAMLAHLDME

Nucleotide

• Length: 318 bp

>T104228 NZ_CP028808:1851103-1851420 [Burkholderia contaminans]
ATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGGCGTCTTCGACACCTGGTTTGCCGGCCTGCAGGATCGCGT
CGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTCTCGATGGGCAATCCGGGCGACTGGAAGTCCGCCGGCTCGCCGG
TCGTCGAGATGCGGATCGACCACGGCCCGGGCTATCGCGTCTACTACGTCCGGCGCGGAACGATCTGGGTCATCCTGCTC
TGCGGCGGCGATAAATCGTCGCAACAGGCCGACATCCGCGCCGCGCACGCGATGCTCGCGCATCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10649.01 Da
• Isoelectric Point: 6.3217

>AT104228 WP_039344756.1 NZ_CP028808:1851424-1851723 [Burkholderia contaminans]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGSKAHA

Nucleotide

• Length: 300 bp

>AT104228 NZ_CP028808:1851424-1851723 [Burkholderia contaminans]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGATCATCTGAAAACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCGCGTGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCCAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCTCGAAAGCGCACGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A1J9PWC2