Detailed information of TA system TA104223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5292330..5292961
• Replicon: chromosome
• Accession: NZ_CP028806
• Organism: Klebsiella pneumoniae strain WCHKP7E2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: R4Y4A3
• Locus tag: B7D49_RS29825
• Protein ID: WP_002882817.1
• Coordinates: 5292330..5292713 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B7D49_RS29830
• Protein ID: WP_002882814.1
• Coordinates: 5292713..5292961 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D49_RS29810	5289696..5290598	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
B7D49_RS29815	5290595..5291230	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
B7D49_RS29820	5291227..5292156	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
B7D49_RS29825	5292330..5292713	-	384	WP_002882817.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D49_RS29830	5292713..5292961	-	249	WP_002882814.1	ribbon-helix-helix domain-containing protein	Antitoxin
B7D49_RS29835	5293160..5294077	+	918	WP_002882812.1	alpha/beta hydrolase	-
B7D49_RS29840	5294091..5295080	-	990	WP_002882811.1	fatty acid biosynthesis protein FabY	-
B7D49_RS29845	5295077..5295514	-	438	WP_002882809.1	D-tyrosyl-tRNA(Tyr) deacylase	-
B7D49_RS29850	5295511..5296371	-	861	WP_002882807.1	virulence factor BrkB family protein	-
B7D49_RS29855	5296365..5296964	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (18-49)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T104223 WP_002882817.1 NZ_CP028806:c5292713-5292330 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T104223 NZ_CP028806:c5292713-5292330 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9786.10 Da
• Isoelectric Point: 4.3509

>AT104223 WP_002882814.1 NZ_CP028806:c5292961-5292713 [Klebsiella pneumoniae]
MSMMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRK
EW

Nucleotide

• Length: 249 bp

>AT104223 NZ_CP028806:c5292961-5292713 [Klebsiella pneumoniae]
ATGAGCATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCT
CAAAGTGCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTA
AAGATACCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAG
GAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GPK8