Detailed information of TA system TA104206

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 7821..8464
• Replicon: plasmid pKPC2_085072
• Accession: NZ_CP028805
• Organism: Klebsiella pneumoniae strain WCHKP7E2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: B7D49_RS02270
• Protein ID: WP_001044770.1
• Coordinates: 7821..8237 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: B7D49_RS02275
• Protein ID: WP_001261282.1
• Coordinates: 8234..8464 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D49_RS02230	2829..3017	+	189	WP_045351231.1	hypothetical protein	-
B7D49_RS02235	2986..3673	+	688	Protein_4	IS3 family transposase	-
B7D49_RS02240	3600..4037	-	438	WP_039065064.1	hypothetical protein	-
B7D49_RS02245	4331..5854	+	1524	WP_000587107.1	group II intron reverse transcriptase/maturase	-
B7D49_RS02250	5935..6114	+	180	Protein_7	IS3 family transposase	-
B7D49_RS02255	6396..6524	+	129	Protein_8	IS110 family transposase	-
B7D49_RS02260	6538..7235	-	698	Protein_9	IS1 family transposase	-
B7D49_RS02265	7289..7747	-	459	Protein_10	ATP-dependent endonuclease	-
B7D49_RS02270	7821..8237	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7D49_RS02275	8234..8464	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B7D49_RS02280	8448..8651	+	204	Protein_13	hypothetical protein	-
B7D49_RS02285	8750..8950	+	201	WP_004201033.1	hypothetical protein	-
B7D49_RS02290	8975..9427	-	453	WP_004201034.1	hypothetical protein	-
B7D49_RS02295	9586..9822	-	237	WP_072065698.1	hypothetical protein	-
B7D49_RS02300	9846..10091	+	246	Protein_17	hypothetical protein	-
B7D49_RS02305	10252..11196	+	945	WP_011977810.1	hypothetical protein	-
B7D49_RS02310	11275..11625	+	351	WP_032738650.1	hypothetical protein	-
B7D49_RS02315	11683..12204	+	522	WP_013214008.1	hypothetical protein	-
B7D49_RS02320	12250..12453	+	204	WP_011977813.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..131028	131028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T104206 WP_001044770.1 NZ_CP028805:c8237-7821 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T104206 NZ_CP028805:c8237-7821 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT104206 WP_001261282.1 NZ_CP028805:c8464-8234 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT104206 NZ_CP028805:c8464-8234 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3