Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 129518..130254 Replicon plasmid pCMY2_085072
Accession NZ_CP028804
Organism Klebsiella pneumoniae strain WCHKP7E2

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag B7D49_RS00995 Protein ID WP_003026803.1
Coordinates 129518..130000 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag B7D49_RS01000 Protein ID WP_003026799.1
Coordinates 129988..130254 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D49_RS00980 125155..126117 + 963 WP_004152113.1 M48 family metalloprotease -
B7D49_RS00985 126205..127459 + 1255 Protein_164 IS3 family transposase -
B7D49_RS00990 127960..129306 - 1347 WP_020314316.1 ISNCY family transposase -
B7D49_RS00995 129518..130000 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
B7D49_RS01000 129988..130254 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
B7D49_RS01005 130430..130684 - 255 WP_004152108.1 hypothetical protein -
B7D49_RS01010 130760..131017 - 258 WP_004152107.1 hypothetical protein -
B7D49_RS01015 131066..131269 - 204 WP_004152106.1 hemolysin expression modulator Hha -
B7D49_RS01020 131303..131671 - 369 WP_004152105.1 hypothetical protein -
B7D49_RS01025 131715..132209 - 495 WP_004152104.1 DNA-binding protein -
B7D49_RS01030 132240..132815 - 576 WP_004152103.1 hypothetical protein -
B7D49_RS01035 132803..133072 - 270 WP_004152102.1 hypothetical protein -
B7D49_RS01040 133430..133780 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
B7D49_RS01045 133830..134192 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / aph(3')-Ia / aac(3)-IIa / erm(B) - 1..323934 323934


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T104205 WP_003026803.1 NZ_CP028804:c130000-129518 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T104205 NZ_CP028804:c130000-129518 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT104205 WP_003026799.1 NZ_CP028804:c130254-129988 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT104205 NZ_CP028804:c130254-129988 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References