Detailed information of TA system TA104203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1804..2278
• Replicon: plasmid p3_085072
• Accession: NZ_CP028803
• Organism: Klebsiella pneumoniae strain WCHKP7E2

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: B7D49_RS00140
• Protein ID: WP_094620559.1
• Coordinates: 2012..2278 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: B7D49_RS00135
• Protein ID: WP_064169727.1
• Coordinates: 1804..2028 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D49_RS00120	115..306	+	192	WP_000166328.1	Rop family plasmid primer RNA-binding protein	-
B7D49_RS00125	306..728	+	423	WP_094620558.1	helix-turn-helix domain-containing protein	-
B7D49_RS00130	1222..1422	+	201	WP_094310467.1	hypothetical protein	-
B7D49_RS00135	1804..2028	+	225	WP_064169727.1	TraY domain-containing protein	Antitoxin
B7D49_RS00140	2012..2278	+	267	WP_094620559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7D49_RS00145	3773..3996	-	224	Protein_5	hypothetical protein	-
B7D49_RS00150	4170..4424	-	255	Protein_6	hypothetical protein	-
B7D49_RS00155	4329..4520	+	192	WP_074193392.1	hypothetical protein	-
B7D49_RS00160	4771..4962	+	192	WP_064169723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5897	5897

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10438.12 Da
• Isoelectric Point: 9.5215

>T104203 WP_094620559.1 NZ_CP028803:2012-2278 [Klebsiella pneumoniae]
MVWMINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRHSGKPLKGELGEFWRYRVGDYRILCEIRDDELIILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T104203 NZ_CP028803:2012-2278 [Klebsiella pneumoniae]
TTGGTCTGGATGATTAACTATTCCGACCGAGCGCTCAAGTCGTTACGTAAGATGGACAAGCAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGTATTGCCGTTGCCGCAGATCCACGCCACTCAGGTAAGCCGCTTAAAGGTGAGCTTGGAGAAT
TCTGGCGCTATCGAGTTGGCGATTATCGCATCCTATGCGAGATCAGGGACGACGAGCTGATTATTCTGGCTGCTACCATT
GGCCACCGACGTGAAGTCTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8466.49 Da
• Isoelectric Point: 4.8182

>AT104203 WP_064169727.1 NZ_CP028803:1804-2028 [Klebsiella pneumoniae]
MLAIRLSDEIESRLNLLAKQTGRTKTFYAREAILTHLDDLEDYYLSAETVARVRRGDEVVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT104203 NZ_CP028803:1804-2028 [Klebsiella pneumoniae]
ATGTTGGCTATCAGATTGTCTGACGAGATTGAGTCCCGTCTAAACTTGCTTGCGAAGCAAACAGGTAGGACTAAAACGTT
CTATGCGCGTGAAGCGATCCTGACGCATCTGGATGATCTTGAAGATTATTATCTTTCAGCAGAAACTGTGGCACGTGTTC
GCCGTGGGGATGAAGTTGTACATTCGTCTGAAGACGTGAGGAAGTCGCTTGGTCTGGATGATTAA