Detailed information of TA system TA104199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 21802..22327
• Replicon: plasmid p1_010059
• Accession: NZ_CP028798
• Organism: Acinetobacter junii strain WCHAJ59

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: CDG57_RS00150
• Protein ID: WP_005130374.1
• Coordinates: 21802..22089 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: CDG57_RS00155
• Protein ID: WP_005130375.1
• Coordinates: 22079..22327 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG57_RS00110	17293..17565	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
CDG57_RS00115	17558..17878	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDG57_RS00120	18044..18583	-	540	WP_002055813.1	hypothetical protein	-
CDG57_RS00125	18608..19447	-	840	WP_001058024.1	alpha/beta fold hydrolase	-
CDG57_RS00130	19540..20130	+	591	WP_000058345.1	TetR/AcrR family transcriptional regulator	-
CDG57_RS00140	20433..20999	-	567	WP_000678311.1	recombinase family protein	-
CDG57_RS00145	21492..21641	+	150	WP_079878061.1	DUF4113 domain-containing protein	-
CDG57_RS00150	21802..22089	-	288	WP_005130374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG57_RS00155	22079..22327	-	249	WP_005130375.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CDG57_RS00160	22330..22755	-	426	WP_005130376.1	hypothetical protein	-
CDG57_RS00165	22838..22906	-	69	Protein_32	helix-turn-helix domain-containing protein	-
CDG57_RS00170	23022..23261	-	240	WP_005130377.1	hypothetical protein	-
CDG57_RS00175	23258..23887	-	630	WP_005130378.1	AAA family ATPase	-
CDG57_RS00180	24338..25252	+	915	WP_004843977.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1..33869	33869

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11146.00 Da
• Isoelectric Point: 10.1961

>T104199 WP_005130374.1 NZ_CP028798:c22089-21802 [Acinetobacter junii]
MTYKLDFLEEALAEWNKLNPSIKQPLKKKLIKVLENPRIPKNKLPGHPNRYKIKLRSVGYRLVYEVIDDEVIFIVIAVGR
RENNAVYDEANSRHS

Nucleotide

• Length: 288 bp

>T104199 NZ_CP028798:c22089-21802 [Acinetobacter junii]
ATGACCTATAAGTTAGACTTTTTAGAAGAAGCATTAGCAGAATGGAATAAGCTTAATCCAAGTATTAAGCAACCATTGAA
GAAGAAACTGATCAAGGTACTAGAGAATCCTCGCATTCCCAAGAATAAGCTCCCAGGACATCCTAACCGCTACAAGATCA
AACTTCGCAGTGTTGGTTATCGTTTGGTTTATGAAGTGATTGATGATGAGGTTATTTTCATCGTCATTGCCGTAGGCAGA
CGTGAAAACAATGCTGTGTATGATGAAGCCAACTCTCGACATTCATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9258.77 Da
• Isoelectric Point: 5.0491

>AT104199 WP_005130375.1 NZ_CP028798:c22327-22079 [Acinetobacter junii]
MTQLIHARMTASITELKKSPMDTVLAGKGEPVAILNRNIPAFYCVPADLYETMMEQLEDLELNKIADARANQKRIRVNID
DL

Nucleotide

• Length: 249 bp

>AT104199 NZ_CP028798:c22327-22079 [Acinetobacter junii]
ATGACTCAATTAATCCATGCACGAATGACTGCAAGTATTACTGAACTCAAAAAGAGTCCGATGGATACAGTGTTAGCAGG
TAAAGGTGAACCTGTAGCGATCCTTAATCGTAATATACCAGCGTTTTACTGTGTTCCGGCTGATTTGTATGAAACCATGA
TGGAACAACTGGAAGACCTGGAACTCAACAAAATTGCAGATGCTCGTGCGAATCAAAAACGCATTCGAGTAAATATTGAT
GACCTATAA