Detailed information of TA system TA104197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 13587..14209
• Replicon: plasmid p1_010059
• Accession: NZ_CP028798
• Organism: Acinetobacter junii strain WCHAJ59

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A7T4PB98
• Locus tag: CDG57_RS00095
• Protein ID: WP_012268403.1
• Coordinates: 13814..14209 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: N9M3S1
• Locus tag: CDG57_RS00090
• Protein ID: WP_005155998.1
• Coordinates: 13587..13817 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG57_RS00055	9169..9456	-	288	WP_005134734.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDG57_RS00060	9446..9634	-	189	WP_004977490.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CDG57_RS00065	9703..10407	-	705	WP_004676054.1	IS6 family transposase	-
CDG57_RS00070	10554..10856	+	303	WP_004676056.1	hypothetical protein	-
CDG57_RS00075	11080..12267	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
CDG57_RS00080	12333..12974	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
CDG57_RS00090	13587..13817	+	231	WP_005155998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CDG57_RS00095	13814..14209	+	396	WP_012268403.1	type II toxin-antitoxin system VapC family toxin	Toxin
CDG57_RS00100	14382..15266	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
CDG57_RS00105	15322..16797	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
CDG57_RS00110	17293..17565	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
CDG57_RS00115	17558..17878	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CDG57_RS00120	18044..18583	-	540	WP_002055813.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1..33869	33869
	-	inside	IScluster/Tn	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1802..16797	14995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14907.18 Da
• Isoelectric Point: 6.2253

>T104197 WP_012268403.1 NZ_CP028798:13814-14209 [Acinetobacter junii]
MIYLLDTNICIYVINNKPQHVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKQALNKFLSPLEILPYDEQAIWHYA
KLRQDLQSTGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Nucleotide

• Length: 396 bp

>T104197 NZ_CP028798:13814-14209 [Acinetobacter junii]
ATGATTTACCTCTTAGACACAAATATTTGTATCTATGTCATCAACAATAAGCCTCAACACGTTTTTGAACGCTTTAAGCA
ATATCAACTCGGCCAGCTTGCGATTTCAAGTATCACGGCATCTGAGCTGGCTTTCGGTGTAGAGAAGTCTGGATCTGAAC
GCAATAAACAGGCATTAAACAAGTTTTTGTCACCTTTAGAGATTCTTCCTTACGATGAACAAGCAATTTGGCATTACGCC
AAATTACGCCAAGATCTTCAATCCACAGGTAAACCGATTGGCAGCTTAGATATGCTGATCGCTGCTCATGCTTTGGCATT
GGATGTTGTATTAGTAACGAACAATACAAAAGAATTTGAACGGATTGATGGTCTAAAGCTAGAGAATTGGGTCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8851.09 Da
• Isoelectric Point: 4.5843

>AT104197 WP_005155998.1 NZ_CP028798:13587-13817 [Acinetobacter junii]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDIMLEAINEFEVGFQLERDDQGEQFREEFR

Nucleotide

• Length: 231 bp

>AT104197 NZ_CP028798:13587-13817 [Acinetobacter junii]
ATGGAAATTGCGAAAGTATTTCAAACTGGTCGCAGTCAAGCGGTTCGCTTGCCTAAAGCCTTTCGCTTTAATGGCACGGA
AGTGGCCATCAAAAGCTTCGGGCGTGGTGTATTACTCATGCCCATCGACAATCCTTGGGATATCATGTTAGAAGCCATCA
ATGAGTTTGAGGTTGGATTCCAGTTAGAGCGAGATGATCAAGGCGAACAGTTCCGTGAGGAGTTCAGATGA

Structures

Toxin

• AlphaFold DB: A0A7T4PB98

Antitoxin

• AlphaFold DB: A0A0A8TT21