Detailed information of TA system TA104196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 9169..9634
• Replicon: plasmid p1_010059
• Accession: NZ_CP028798
• Organism: Acinetobacter junii strain WCHAJ59

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R9B9L0
• Locus tag: CDG57_RS00055
• Protein ID: WP_005134734.1
• Coordinates: 9169..9456 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9CS90
• Locus tag: CDG57_RS00060
• Protein ID: WP_004977490.1
• Coordinates: 9446..9634 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDG57_RS00025	4475..4702	-	228	WP_000248278.1	hypothetical protein	-
CDG57_RS00030	4726..4917	+	192	WP_000951934.1	hypothetical protein	-
CDG57_RS00035	5399..5941	-	543	WP_000587837.1	AAA family ATPase	-
CDG57_RS00040	5954..6814	-	861	WP_000557454.1	aminoglycoside N-acetyltransferase AAC(3)-IId	-
CDG57_RS00045	7106..7684	+	579	WP_004676046.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
CDG57_RS00050	7700..9004	+	1305	WP_087540684.1	Y-family DNA polymerase	-
CDG57_RS00055	9169..9456	-	288	WP_005134734.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDG57_RS00060	9446..9634	-	189	WP_004977490.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
CDG57_RS00065	9703..10407	-	705	WP_004676054.1	IS6 family transposase	-
CDG57_RS00070	10554..10856	+	303	WP_004676056.1	hypothetical protein	-
CDG57_RS00075	11080..12267	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
CDG57_RS00080	12333..12974	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
CDG57_RS00090	13587..13817	+	231	WP_005155998.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CDG57_RS00095	13814..14209	+	396	WP_012268403.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1..33869	33869
	-	inside	IScluster/Tn	aac(3)-IId / tet(39) / mph(E) / msr(E)	-	1802..16797	14995

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11073.89 Da
• Isoelectric Point: 10.1961

>T104196 WP_005134734.1 NZ_CP028798:c9456-9169 [Acinetobacter junii]
MTYKLDFLEEALAEWNKLNPSIKQPLKKKLIKVLENPRIPKNKLSGHPNRYKIKLRSIGYRLVYEVIDDEVVVIVIAVGR
RENNAVYDDANSRHS

Nucleotide

• Length: 288 bp

>T104196 NZ_CP028798:c9456-9169 [Acinetobacter junii]
ATGACCTATAAGTTAGACTTTTTAGAAGAAGCATTAGCAGAATGGAATAAGCTGAATCCAAGTATTAAGCAGCCATTGAA
GAAAAAGTTGATCAAGGTTTTGGAGAATCCTCGTATTCCCAAGAATAAGCTGTCAGGGCATCCTAACCGCTACAAGATCA
AGCTTCGCAGCATTGGTTATCGTCTGGTTTATGAAGTCATTGATGATGAGGTAGTTGTCATCGTGATTGCTGTAGGCAGA
CGTGAAAACAATGCCGTCTATGATGATGCAAACTCTCGTCATTCATAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7008.03 Da
• Isoelectric Point: 4.2677

>AT104196 WP_004977490.1 NZ_CP028798:c9634-9446 [Acinetobacter junii]
MDTVLAGKGEPVAILNRNTPAFYCVPADLYETMMEQLEDLELNKIADARANQKRIRVNIDDL

Nucleotide

• Length: 189 bp

>AT104196 NZ_CP028798:c9634-9446 [Acinetobacter junii]
ATGGATACAGTGTTGGCAGGCAAAGGCGAACCTGTCGCAATCCTCAATCGTAATACACCAGCGTTTTACTGTGTTCCAGC
TGATCTGTATGAAACGATGATGGAACAACTAGAAGACCTAGAACTGAATAAGATTGCAGATGCTCGTGCGAATCAGAAAC
GCATTCGAGTAAATATTGATGACCTATAA

Structures

Toxin

• AlphaFold DB: R9B9L0

Antitoxin

• AlphaFold DB: A0A7T4PB15