Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71240..71883 Replicon plasmid pKPC2_040035
Accession NZ_CP028796
Organism Klebsiella pneumoniae strain WCHKP040035

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C6M51_RS00880 Protein ID WP_001044770.1
Coordinates 71240..71656 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C6M51_RS00885 Protein ID WP_001261282.1
Coordinates 71653..71883 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M51_RS00860 67343..67615 - 273 Protein_105 IS5/IS1182 family transposase -
C6M51_RS00865 67644..68341 - 698 Protein_106 IS1 family transposase -
C6M51_RS00870 68597..69619 - 1023 WP_000361404.1 helicase UvrD -
C6M51_RS00875 69604..71166 - 1563 WP_004206609.1 AAA family ATPase -
C6M51_RS00880 71240..71656 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M51_RS00885 71653..71883 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6M51_RS00890 71867..72301 + 435 WP_015493090.1 hypothetical protein -
C6M51_RS00895 72462..73406 + 945 WP_011977810.1 hypothetical protein -
C6M51_RS00900 73485..73835 + 351 WP_032738650.1 hypothetical protein -
C6M51_RS00905 73893..74414 + 522 WP_013214008.1 hypothetical protein -
C6M51_RS00910 74460..74663 + 204 WP_011977813.1 hypothetical protein -
C6M51_RS00915 74693..75697 + 1005 WP_011977814.1 hypothetical protein -
C6M51_RS00920 75881..76660 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2 - 1..112467 112467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T104173 WP_001044770.1 NZ_CP028796:c71656-71240 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T104173 NZ_CP028796:c71656-71240 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT104173 WP_001261282.1 NZ_CP028796:c71883-71653 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT104173 NZ_CP028796:c71883-71653 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References