104153

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 207640..208162 Replicon plasmid pOXA1_020030
Accession NZ_CP028791
Organism Klebsiella pneumoniae strain WCHKP020030

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag C6M02_RS02240 Protein ID WP_004181778.1
Coordinates 207878..208162 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag C6M02_RS02235 Protein ID WP_004181777.1
Coordinates 207640..207888 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M02_RS02210 202666..203019 - 354 WP_004181774.1 hypothetical protein -
C6M02_RS02215 203164..203640 - 477 WP_108458837.1 hypothetical protein -
C6M02_RS02220 203629..204552 + 924 WP_012579081.1 IS5 family transposase -
C6M02_RS02225 204608..205216 - 609 Protein_257 hypothetical protein -
C6M02_RS02230 205550..207349 - 1800 WP_004181776.1 ATP-dependent helicase -
C6M02_RS02235 207640..207888 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
C6M02_RS02240 207878..208162 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6M02_RS02245 208179..208280 - 102 Protein_261 IS200/IS605 family transposase -
C6M02_RS02250 208316..209542 + 1227 WP_040209639.1 transposase -
C6M02_RS02255 209813..210037 - 225 Protein_263 transposase -
C6M02_RS02260 210116..210544 - 429 WP_077257669.1 IS200/IS605 family transposase -
C6M02_RS02265 210580..211767 + 1188 WP_040209642.1 transposase -
C6M02_RS02270 211866..212961 - 1096 Protein_266 conjugal transfer protein TraG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr iucA / iucB / iucC / iucD / iutA / rmpA2 1..288222 288222
- inside IScluster/Tn - - 203584..211767 8183


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T104153 WP_004181778.1 NZ_CP028791:207878-208162 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T104153 NZ_CP028791:207878-208162 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT104153 WP_004181777.1 NZ_CP028791:207640-207888 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT104153 NZ_CP028791:207640-207888 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References