Detailed information of TA system TA104151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 113692..114362
• Replicon: plasmid pOXA1_020030
• Accession: NZ_CP028791
• Organism: Klebsiella pneumoniae strain WCHKP020030

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C6M02_RS01645
• Protein ID: WP_004213072.1
• Coordinates: 113692..114135 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C6M02_RS01650
• Protein ID: WP_004213073.1
• Coordinates: 114132..114362 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M02_RS01595	109099..109374	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
C6M02_RS01600	109437..109928	+	492	WP_011251327.1	DM13 domain-containing protein	-
C6M02_RS01605	109977..110897	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
C6M02_RS01610	110988..111191	+	204	WP_004213936.1	hypothetical protein	-
C6M02_RS01615	111119..111394	+	276	WP_004213938.1	GAF domain-containing protein	-
C6M02_RS01620	111459..111652	-	194	Protein_136	hypothetical protein	-
C6M02_RS01625	111726..111908	-	183	WP_071591913.1	hypothetical protein	-
C6M02_RS01630	112201..112548	-	348	WP_077268747.1	hypothetical protein	-
C6M02_RS01635	112965..113258	+	294	Protein_139	IS3 family transposase	-
C6M02_RS01640	113262..113678	+	417	Protein_140	DUF3644 domain-containing protein	-
C6M02_RS01645	113692..114135	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C6M02_RS01650	114132..114362	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
C6M02_RS01655	114970..116103	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C6M02_RS01660	116119..116412	+	294	WP_004213076.1	hypothetical protein	-
C6M02_RS01665	116402..116608	-	207	WP_004213077.1	hypothetical protein	-
C6M02_RS01670	116960..117250	+	291	WP_004213078.1	hypothetical protein	-
C6M02_RS01675	117240..118139	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	iucA / iucB / iucC / iucD / iutA / rmpA2	1..288222	288222

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T104151 WP_004213072.1 NZ_CP028791:c114135-113692 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T104151 NZ_CP028791:c114135-113692 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT104151 WP_004213073.1 NZ_CP028791:c114362-114132 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT104151 NZ_CP028791:c114362-114132 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620