Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 34906..35816 Replicon plasmid pOXA1_020030
Accession NZ_CP028791
Organism Klebsiella pneumoniae strain WCHKP020030

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag C6M02_RS01180 Protein ID WP_004026354.1
Coordinates 35346..35816 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag C6M02_RS01175 Protein ID WP_004181895.1
Coordinates 34906..35349 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M02_RS01150 31291..31542 + 252 WP_009483812.1 hypothetical protein -
C6M02_RS01155 31542..33026 + 1485 WP_004152765.1 group II intron reverse transcriptase/maturase -
C6M02_RS01160 32942..33121 + 180 WP_077253892.1 hypothetical protein -
C6M02_RS01165 33112..33534 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
C6M02_RS01170 33534..34805 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
C6M02_RS01175 34906..35349 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
C6M02_RS01180 35346..35816 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
C6M02_RS01185 35926..36186 - 261 WP_004026352.1 hypothetical protein -
C6M02_RS01190 36757..36900 - 144 Protein_50 IS481 family transposase -
C6M02_RS01195 36909..37067 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
C6M02_RS01200 37670..38160 + 491 Protein_52 hypothetical protein -
C6M02_RS01205 38288..38644 + 357 WP_071528222.1 hypothetical protein -
C6M02_RS01210 38842..39219 - 378 WP_004181901.1 hypothetical protein -
C6M02_RS01215 39400..39588 - 189 WP_072196720.1 hypothetical protein -
C6M02_RS01220 39545..39841 + 297 WP_032409777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr iucA / iucB / iucC / iucD / iutA / rmpA2 1..288222 288222


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T104150 WP_004026354.1 NZ_CP028791:35346-35816 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T104150 NZ_CP028791:35346-35816 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT104150 WP_004181895.1 NZ_CP028791:34906-35349 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT104150 NZ_CP028791:34906-35349 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References