Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4567784..4568281 Replicon chromosome
Accession NZ_CP028772
Organism Escherichia coli strain A2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY53_RS22315 Protein ID WP_001173343.1
Coordinates 4567784..4567957 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag AOY53_RS22320 Protein ID WP_000398619.1
Coordinates 4567985..4568281 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY53_RS22305 (4564454) 4564454..4565911 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
AOY53_RS22310 (4566148) 4566148..4567665 + 1518 WP_001295074.1 lysine--tRNA ligase -
AOY53_RS22315 (4567784) 4567784..4567957 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY53_RS22320 (4567985) 4567985..4568281 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY53_RS22325 (4568508) 4568508..4568780 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY53_RS22330 (4568792) 4568792..4569022 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
AOY53_RS22335 (4569203) 4569203..4570834 + 1632 WP_001216460.1 sensor histidine kinase -
AOY53_RS22340 (4570831) 4570831..4571550 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4527658..4568281 40623


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T104106 WP_001173343.1 NZ_CP028772:c4567957-4567784 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T104106 NZ_CP028772:c4567957-4567784 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT104106 WP_000398619.1 NZ_CP028772:c4568281-4567985 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT104106 NZ_CP028772:c4568281-4567985 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References