Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4551648..4552449 | Replicon | chromosome |
| Accession | NZ_CP028772 | ||
| Organism | Escherichia coli strain A2 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A3W5RL53 |
| Locus tag | AOY53_RS22245 | Protein ID | WP_041124173.1 |
| Coordinates | 4552072..4552449 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A3W5RLB4 |
| Locus tag | AOY53_RS22240 | Protein ID | WP_041124172.1 |
| Coordinates | 4551648..4552025 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AOY53_RS22205 (4547549) | 4547549..4548229 | + | 681 | WP_001282912.1 | WYL domain-containing protein | - |
| AOY53_RS22210 (4548380) | 4548380..4549057 | + | 678 | WP_021559490.1 | hypothetical protein | - |
| AOY53_RS22215 (4549063) | 4549063..4549296 | + | 234 | WP_001278287.1 | DUF905 family protein | - |
| AOY53_RS22220 (4549395) | 4549395..4550213 | + | 819 | WP_240760637.1 | DUF932 domain-containing protein | - |
| AOY53_RS22225 (4550305) | 4550305..4550790 | + | 486 | WP_041124170.1 | antirestriction protein | - |
| AOY53_RS22230 (4550806) | 4550806..4551282 | + | 477 | WP_021559500.1 | RadC family protein | - |
| AOY53_RS22235 (4551351) | 4551351..4551572 | + | 222 | WP_000692307.1 | DUF987 domain-containing protein | - |
| AOY53_RS22240 (4551648) | 4551648..4552025 | + | 378 | WP_041124172.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| AOY53_RS22245 (4552072) | 4552072..4552449 | + | 378 | WP_041124173.1 | TA system toxin CbtA family protein | Toxin |
| AOY53_RS22250 (4552446) | 4552446..4552934 | + | 489 | WP_041124174.1 | DUF5983 family protein | - |
| AOY53_RS22255 (4552946) | 4552946..4553143 | + | 198 | WP_000839249.1 | DUF957 domain-containing protein | - |
| AOY53_RS22260 (4553228) | 4553228..4554073 | + | 846 | WP_053289820.1 | DUF4942 domain-containing protein | - |
| AOY53_RS22265 (4554182) | 4554182..4554760 | + | 579 | WP_053289821.1 | hypothetical protein | - |
| AOY53_RS22270 (4554852) | 4554852..4555232 | + | 381 | WP_001171554.1 | transposase | - |
| AOY53_RS22275 (4555229) | 4555229..4555576 | + | 348 | WP_000612589.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| AOY53_RS22280 (4555626) | 4555626..4557164 | + | 1539 | WP_000998048.1 | IS66-like element ISEc8 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4527658..4568281 | 40623 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13991.90 Da Isoelectric Point: 7.3225
>T104105 WP_041124173.1 NZ_CP028772:4552072-4552449 [Escherichia coli]
MNTLPDTHVRETSGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR
MNTLPDTHVRETSGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR
Download Length: 378 bp
>T104105 NZ_CP028772:4552072-4552449 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGACATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGTACGGAGGCAAAACGATGA
ATGAATACATTACCCGACACTCACGTACGGGAGACATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGTACGGAGGCAAAACGATGA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13752.57 Da Isoelectric Point: 6.2021
>AT104105 WP_041124172.1 NZ_CP028772:4551648-4552025 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRASIRGRFSDADAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
Download Length: 378 bp
>AT104105 NZ_CP028772:4551648-4552025 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCAGGT
TCAGCGACGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGTCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCAGTATCAGAGGCAGGT
TCAGCGACGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGTCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5RL53 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3W5RLB4 |