Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3671313..3671931 Replicon chromosome
Accession NZ_CP028772
Organism Escherichia coli strain A2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY53_RS17985 Protein ID WP_001291435.1
Coordinates 3671713..3671931 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY53_RS17980 Protein ID WP_000344800.1
Coordinates 3671313..3671687 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY53_RS17970 (3666402) 3666402..3667595 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AOY53_RS17975 (3667618) 3667618..3670767 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
AOY53_RS17980 (3671313) 3671313..3671687 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY53_RS17985 (3671713) 3671713..3671931 + 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY53_RS17990 (3672102) 3672102..3672653 + 552 WP_000102564.1 maltose O-acetyltransferase -
AOY53_RS17995 (3672769) 3672769..3673239 + 471 WP_000136192.1 YlaC family protein -
AOY53_RS18000 (3673403) 3673403..3674953 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY53_RS18005 (3674995) 3674995..3675348 - 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY53_RS18015 (3675727) 3675727..3676038 + 312 WP_000409911.1 MGMT family protein -
AOY53_RS18020 (3676069) 3676069..3676641 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T104101 WP_001291435.1 NZ_CP028772:3671713-3671931 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T104101 NZ_CP028772:3671713-3671931 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT104101 WP_000344800.1 NZ_CP028772:3671313-3671687 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT104101 NZ_CP028772:3671313-3671687 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References