Detailed information of TA system TA104100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2615728..2616366
• Replicon: chromosome
• Accession: NZ_CP028772
• Organism: Escherichia coli strain A2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AOY53_RS12805
• Protein ID: WP_000813794.1
• Coordinates: 2616190..2616366 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AOY53_RS12800
• Protein ID: WP_001270285.1
• Coordinates: 2615728..2616144 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY53_RS12780 (2611436)	2611436..2612230	-	795	WP_240760607.1	ABC transporter permease	-
AOY53_RS12785 (2612220)	2612220..2613161	-	942	WP_001251315.1	ABC transporter permease	-
AOY53_RS12790 (2613162)	2613162..2613998	-	837	WP_072025245.1	extracellular solute-binding protein	-
AOY53_RS12795 (2614243)	2614243..2615649	-	1407	WP_000760594.1	PLP-dependent aminotransferase family protein	-
AOY53_RS12800 (2615728)	2615728..2616144	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AOY53_RS12805 (2616190)	2616190..2616366	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AOY53_RS12810 (2616588)	2616588..2616818	+	231	WP_000494244.1	YncJ family protein	-
AOY53_RS12815 (2616910)	2616910..2618871	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AOY53_RS12820 (2618944)	2618944..2619480	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AOY53_RS12825 (2619533)	2619533..2620747	+	1215	WP_071601296.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2620787..2621935	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T104100 WP_000813794.1 NZ_CP028772:c2616366-2616190 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T104100 NZ_CP028772:c2616366-2616190 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT104100 WP_001270285.1 NZ_CP028772:c2616144-2615728 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT104100 NZ_CP028772:c2616144-2615728 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB