Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1476900..1477525 Replicon chromosome
Accession NZ_CP028772
Organism Escherichia coli strain A2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag AOY53_RS07245 Protein ID WP_000911329.1
Coordinates 1477127..1477525 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag AOY53_RS07240 Protein ID WP_000450524.1
Coordinates 1476900..1477127 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY53_RS07215 (1472702) 1472702..1473172 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
AOY53_RS07220 (1473172) 1473172..1473744 - 573 WP_000176191.1 glycine cleavage system transcriptional repressor -
AOY53_RS07225 (1473890) 1473890..1474768 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
AOY53_RS07230 (1474785) 1474785..1475819 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
AOY53_RS07235 (1476032) 1476032..1476745 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
AOY53_RS07240 (1476900) 1476900..1477127 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
AOY53_RS07245 (1477127) 1477127..1477525 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
AOY53_RS07250 (1477672) 1477672..1478535 + 864 WP_001267498.1 neutral zinc metallopeptidase -
AOY53_RS07255 (1478550) 1478550..1480565 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
AOY53_RS07260 (1480639) 1480639..1481337 + 699 WP_000679823.1 esterase -
AOY53_RS07265 (1481447) 1481447..1481647 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T104089 WP_000911329.1 NZ_CP028772:1477127-1477525 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T104089 NZ_CP028772:1477127-1477525 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT104089 WP_000450524.1 NZ_CP028772:1476900-1477127 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT104089 NZ_CP028772:1476900-1477127 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References