Detailed information of TA system TA104088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 1279478..1280903
• Replicon: chromosome
• Accession: NZ_CP028772
• Organism: Escherichia coli strain A2

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: AOY53_RS06245
• Protein ID: WP_024190590.1
• Coordinates: 1279478..1280548 (+)
• Length: 357 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: E6BSF0
• Locus tag: AOY53_RS06250
• Protein ID: WP_000461705.1
• Coordinates: 1280541..1280903 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY53_RS06225 (1274803)	1274803..1275402	+	600	WP_001230356.1	Ail/Lom family outer membrane beta-barrel protein	-
AOY53_RS06230 (1275467)	1275467..1278493	+	3027	WP_240760658.1	prophage tail fiber N-terminal domain-containing protein	-
AOY53_RS06235 (1278493)	1278493..1279077	+	585	WP_023363231.1	tail fiber assembly protein	-
AOY53_RS06240 (1279147)	1279147..1279343	+	197	Protein_1225	recombinase family protein	-
AOY53_RS06245 (1279478)	1279478..1280548	+	1071	WP_024190590.1	type II toxin-antitoxin system RnlA family toxin	Toxin
AOY53_RS06250 (1280541)	1280541..1280903	+	363	WP_000461705.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
AOY53_RS06255 (1281041)	1281041..1281310	-	270	WP_000429347.1	hypothetical protein	-
AOY53_RS06265 (1282188)	1282188..1282670	-	483	WP_000162574.1	SsrA-binding protein SmpB	-
AOY53_RS06270 (1282841)	1282841..1283278	+	438	WP_001341638.1	type II toxin-antitoxin system toxin RatA	-
AOY53_RS06275 (1283268)	1283268..1283558	+	291	WP_001117838.1	RnfH family protein	-
AOY53_RS06280 (1283620)	1283620..1283961	-	342	WP_001203437.1	outer membrane protein assembly factor BamE	-
AOY53_RS06285 (1284110)	1284110..1285771	-	1662	WP_000880910.1	DNA repair protein RecN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (15-112)

Sequences

Toxin

Protein

• Length: 357 a.a.
• Molecular weight: 40475.40 Da
• Isoelectric Point: 7.2332

>T104088 WP_024190590.1 NZ_CP028772:1279478-1280548 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANRNL
GKELADNLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGPYFDKIGSEFILKQQFGRNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Nucleotide

• Length: 1071 bp

>T104088 NZ_CP028772:1279478-1280548 [Escherichia coli]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTCTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCGTAATCTT
GGAAAAGAGTTGGCTGATAATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCCGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCCTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGACGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13304.23 Da
• Isoelectric Point: 4.2386

>AT104088 WP_000461705.1 NZ_CP028772:1280541-1280903 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Nucleotide

• Length: 363 bp

>AT104088 NZ_CP028772:1280541-1280903 [Escherichia coli]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A1X9TDS3