Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 314303..314889 Replicon chromosome
Accession NZ_CP028772
Organism Escherichia coli strain A2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag AOY53_RS01430 Protein ID WP_042014162.1
Coordinates 314303..314533 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AOY53_RS01435 Protein ID WP_000593555.1
Coordinates 314530..314889 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY53_RS01420 (310340) 310340..313075 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
AOY53_RS01425 (313075) 313075..314199 + 1125 WP_001216257.1 ABC transporter permease -
AOY53_RS01430 (314303) 314303..314533 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
AOY53_RS01435 (314530) 314530..314889 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AOY53_RS01440 (315009) 315009..315410 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
AOY53_RS01445 (315416) 315416..316222 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
AOY53_RS01450 (316219) 316219..316983 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
AOY53_RS01455 (316983) 316983..317816 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
AOY53_RS01460 (317813) 317813..318757 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T104083 WP_042014162.1 NZ_CP028772:314303-314533 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T104083 NZ_CP028772:314303-314533 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT104083 WP_000593555.1 NZ_CP028772:314530-314889 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT104083 NZ_CP028772:314530-314889 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References