Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3810696..3811350 Replicon chromosome
Accession NZ_CP028771
Organism Escherichia coli strain A3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag AOY54_RS18380 Protein ID WP_000244781.1
Coordinates 3810696..3811103 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag AOY54_RS18385 Protein ID WP_000354046.1
Coordinates 3811084..3811350 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY54_RS18360 (3806653) 3806653..3808386 - 1734 WP_042020700.1 single-stranded-DNA-specific exonuclease RecJ -
AOY54_RS18365 (3808392) 3808392..3809102 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
AOY54_RS18370 (3809127) 3809127..3810023 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
AOY54_RS18375 (3810135) 3810135..3810656 + 522 WP_001055874.1 flavodoxin FldB -
AOY54_RS18380 (3810696) 3810696..3811103 - 408 WP_000244781.1 protein YgfX Toxin
AOY54_RS18385 (3811084) 3811084..3811350 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
AOY54_RS18390 (3811603) 3811603..3812583 + 981 WP_021513792.1 tRNA-modifying protein YgfZ -
AOY54_RS18395 (3812660) 3812660..3813319 - 660 WP_000250274.1 hemolysin III family protein -
AOY54_RS18400 (3813483) 3813483..3813794 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
AOY54_RS18405 (3813839) 3813839..3815272 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T104081 WP_000244781.1 NZ_CP028771:c3811103-3810696 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T104081 NZ_CP028771:c3811103-3810696 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTAATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTACGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT104081 WP_000354046.1 NZ_CP028771:c3811350-3811084 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT104081 NZ_CP028771:c3811350-3811084 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References