Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1315805..1316423 Replicon chromosome
Accession NZ_CP028771
Organism Escherichia coli strain A3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AOY54_RS06205 Protein ID WP_001291435.1
Coordinates 1315805..1316023 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AOY54_RS06210 Protein ID WP_000344800.1
Coordinates 1316049..1316423 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY54_RS06170 (1311093) 1311093..1311665 + 573 WP_000779831.1 YbaY family lipoprotein -
AOY54_RS06175 (1311696) 1311696..1312007 - 312 WP_000409908.1 MGMT family protein -
AOY54_RS06185 (1312386) 1312386..1312739 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AOY54_RS06190 (1312781) 1312781..1314331 - 1551 WP_089638034.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AOY54_RS06195 (1314495) 1314495..1314965 - 471 WP_000136192.1 YlaC family protein -
AOY54_RS06200 (1315081) 1315081..1315632 - 552 WP_000102539.1 maltose O-acetyltransferase -
AOY54_RS06205 (1315805) 1315805..1316023 - 219 WP_001291435.1 HHA domain-containing protein Toxin
AOY54_RS06210 (1316049) 1316049..1316423 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AOY54_RS06215 (1316968) 1316968..1320117 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
AOY54_RS06220 (1320140) 1320140..1321333 - 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T104064 WP_001291435.1 NZ_CP028771:c1316023-1315805 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T104064 NZ_CP028771:c1316023-1315805 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT104064 WP_000344800.1 NZ_CP028771:c1316423-1316049 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT104064 NZ_CP028771:c1316423-1316049 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References