Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4491154..4491651 Replicon chromosome
Accession NZ_CP028770
Organism Escherichia coli strain A4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag AOY55_RS21610 Protein ID WP_001173343.1
Coordinates 4491154..4491327 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag AOY55_RS21615 Protein ID WP_001312331.1
Coordinates 4491355..4491651 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY55_RS21600 (4487824) 4487824..4489281 + 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -
AOY55_RS21605 (4489518) 4489518..4491035 + 1518 WP_000003805.1 lysine--tRNA ligase -
AOY55_RS21610 (4491154) 4491154..4491327 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
AOY55_RS21615 (4491355) 4491355..4491651 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
AOY55_RS21620 (4491880) 4491880..4492152 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
AOY55_RS21625 (4492164) 4492164..4492394 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
AOY55_RS21630 (4492575) 4492575..4494206 + 1632 WP_001216466.1 sensor histidine kinase -
AOY55_RS21635 (4494203) 4494203..4494922 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T104056 WP_001173343.1 NZ_CP028770:c4491327-4491154 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T104056 NZ_CP028770:c4491327-4491154 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT104056 WP_001312331.1 NZ_CP028770:c4491651-4491355 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT104056 NZ_CP028770:c4491651-4491355 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References