Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4270194..4270770 Replicon chromosome
Accession NZ_CP028770
Organism Escherichia coli strain A4

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag AOY55_RS20605 Protein ID WP_001350372.1
Coordinates 4270483..4270770 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag AOY55_RS20600 Protein ID WP_000063155.1
Coordinates 4270194..4270496 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY55_RS20585 (4266722) 4266722..4268872 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
AOY55_RS20590 (4268990) 4268990..4269193 + 204 WP_000467859.1 YbdD/YjiX family protein -
AOY55_RS20595 (4269204) 4269204..4270160 + 957 WP_001350371.1 GTPase -
AOY55_RS20600 (4270194) 4270194..4270496 - 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
AOY55_RS20605 (4270483) 4270483..4270770 - 288 WP_001350372.1 BrnT family toxin Toxin
AOY55_RS20610 (4270967) 4270967..4272133 + 1167 WP_000800824.1 restriction endonuclease -
AOY55_RS20615 (4272197) 4272197..4273816 + 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
AOY55_RS20620 (4273806) 4273806..4275137 + 1332 WP_000505211.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T104055 WP_001350372.1 NZ_CP028770:c4270770-4270483 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T104055 NZ_CP028770:c4270770-4270483 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT104055 WP_000063155.1 NZ_CP028770:c4270496-4270194 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT104055 NZ_CP028770:c4270496-4270194 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References